U2AF2 antibody
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WB ResetRabbit anti U2AF2
- Product Type
- PrecisionAb Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
- Specificity
- U2AF2
Antibody Quality and Performance Guarantee
| Rabbit anti Human U2AF2 antibody recognizes the splicing factor U2AF 65 kDa subunit, also known as U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 snRNP auxiliary factor large subunit and hU2AF65. Splicing factor U2AF is comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF2 gene encodes the large 65 kDa subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for U2AF2, but the full-length natures of only two have been determined to date (provided by RefSeq, Jul 2008). Rabbit anti Human U2AF2 antibody detects a band of 65 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
|
- Target Species
- Human
- Western Blotting
- Anti U2AF2 detects a band of approximately 65 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Immunogen
- KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human U2AF2
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
- UniProt
- P26368
- Entrez Gene
- U2AF2
- GO Terms
- GO:0000166 nucleotide binding
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0003723 RNA binding
- GO:0005654 nucleoplasm
- GO:0005681 spliceosomal complex
- GO:0006369 termination of RNA polymerase II transcription
- GO:0006406 mRNA export from nucleus
- GO:0019899 enzyme binding
- GO:0031124 mRNA 3'-end processing
- View More GO Terms
VPA00241
151204
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