U2AF2 antibody

100% Secure


Rabbit anti U2AF2

U2AF2 antibody recognizes splicing factor U2AF 65 kDa subunit, also known as U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65 kDa), or U2 snRNP auxiliary factor large subunit.

U2AF2 Antibody with Control Lysate

U2AF2 antibody recognizes splicing factor U2AF 65 kDa subunit, also known as U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65 kDa), or U2 snRNP auxiliary factor large subunit.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00241 WB 100 µl
VPA00241KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human U2AF2 antibody recognizes the splicing factor U2AF 65 kDa subunit, also known as U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 snRNP auxiliary factor large subunit and hU2AF65.

Splicing factor U2AF is comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF2 gene encodes the large 65 kDa subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for U2AF2, but the full-length natures of only two have been determined to date (provided by RefSeq, Jul 2008).

Rabbit anti Human U2AF2 antibody detects a band of 65 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human U2AF2

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P26368 Related reagents
Entrez Gene
U2AF2 Related reagents
GO Terms
GO:0000166 nucleotide binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003723 RNA binding
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0006369 termination of RNA polymerase II transcription
GO:0006406 mRNA export from nucleus
GO:0019899 enzyme binding
GO:0031124 mRNA 3'-end processing
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of U2AF2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti U2AF2 detects a band of approximately 65 kDa in HEK293 cell lysates
Western Blotting
Anti U2AF2 detects a band of approximately 65 kDa in HEK293 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB