U2AF1 antibody
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WB ResetRabbit anti U2AF1
- Product Type
- PrecisionAb Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
- Specificity
- U2AF1
Antibody Quality and Performance Guarantee
| Rabbit anti Human U2AF1 antibody recognizes splicing factor U2AF 35 kDa subunit also known as U2 small nuclear RNA auxillary factor 1, U2 small nuclear ribonucleoprotein auxillary factor 35-KD subunit, U2 snRNP auxiliary factor small subunit or U2(RNU2) small nuclear RNA auxiliary factor binding protein. The U2AF1 gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF1 encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified (provided by RefSeq, Jul 2008). Rabbit anti Human U2AF1 antibody detects a band of 35 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
|
- Target Species
- Human
- Western Blotting
- Rabbit anti U2AF1 detects a band of approximately 35 kDa in Raji cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- TRIS buffered glycine.
- Preservative Stabilisers
- 0.01% Thiomersal
<50% Glycerol - Immunogen
- Recombinant protein encompassing part of the middle region of human U2AF1
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
- UniProt
- Q01081
- Entrez Gene
- U2AF1
- GO Terms
- GO:0000166 nucleotide binding
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0003723 RNA binding
- GO:0005681 spliceosomal complex
- GO:0006369 termination of RNA polymerase II transcription
- GO:0006406 mRNA export from nucleus
- GO:0008270 zinc ion binding
- GO:0015030 Cajal body
- GO:0016607 nuclear speck
- View More GO Terms
- GO:0031124 mRNA 3'-end processing
VPA00733
161118
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