Tspo antibody
Rabbit anti Tspo
TSPO antibody recognizes TSPO, also known as translocator protein, benzodiazepine peripheral binding site, mitochondrial benzodiazepine receptor or peripheral-type benzodiazepine receptor.
Tspo Antibody with Control Lysate
TSPO antibody recognizes TSPO, also known as translocator protein, benzodiazepine peripheral binding site, mitochondrial benzodiazepine receptor or peripheral-type benzodiazepine receptor.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00789 | WB | 100 µl | ||||
| VPA00789KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human TSPO antibody recognizes TSPO, also known as translocator protein, benzodiazepine peripheral binding site, mitochondrial benzodiazepine receptor or peripheral-type benzodiazepine receptor.
Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by TSPO interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein (provided by RefSeq, Feb 2012).
Rabbit anti Human TSPO antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by TSPO interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein (provided by RefSeq, Feb 2012).
Rabbit anti Human TSPO antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti TSPO antibody recognizes a band of approximately 18 kDa in C2C12 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide directed towards the C terminal region of human TSPO
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg C2C12 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P30536 Related reagents
- Entrez Gene
- TSPO Related reagents
- GO Terms
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0005741 mitochondrial outer membrane
- GO:0006626 protein targeting to mitochondrion
- GO:0006783 heme biosynthetic process
- GO:0006820 anion transport
- GO:0008202 steroid metabolic process
- GO:0008283 cell proliferation
- GO:0008503 benzodiazepine receptor activity
- GO:0015485 cholesterol binding
- GO:0032374 regulation of cholesterol transport
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Tspo antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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