TRPC6 antibody

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Rabbit anti TRPC6

Rabbit anti human TRPC6 antibody recognizes short transient receptor potential channel 6, also known as TRP-6, focal segmental glomerulosclerosis 2 or transient receptor protein 6. This antibody has been extensively validated for western blotting using whole cell lysates.

TRPC6 Antibody with Control Lysate

Rabbit anti human TRPC6 antibody recognizes short transient receptor potential channel 6, also known as TRP-6, focal segmental glomerulosclerosis 2 or transient receptor protein 6. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00375 WB 100 µl
VPA00375KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human TRPC6 antibody recognizes TRPC6, also known as TRP-6, focal segmental glomerulosclerosis 2, short transient receptor potential cation channel 6 subfamily C, member 6 and transient receptor protein 6.

The protein encoded by the TRPC6 gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in TRPC6 are a cause of focal segmental glomerulosclerosis 2 (FSGS2) (provided by RefSeq, Mar 2009).

Rabbit anti Human TRPC6 antibody detects a band of 106 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti TRPC6 detects a band of approximately 106 kDa in HepG2 cell lysate
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide corresponding to the central region of human TRPC6
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HepG2 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9Y210 Related reagents
Entrez Gene
TRPC6 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0007411 axon guidance
GO:0030168 platelet activation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of TRPC6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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