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Troponin T antibody

Goat anti Troponin T (Cardiac)

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Troponin T

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VPA00199
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Goat anti Human troponin T antibody recognizes troponin T also known as cardiomyopathy, dilated 1D (autosomal dominant), cardiomyopathy, hypertrophic 2 and troponin T cardiac muscle, .

The protein encoded by the TNNT2 gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in TNNT2 have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for TNNT2 undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined (provided by RefSeq, Jul 2008).

Goat anti Human troponin T antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti troponin T detects a band of approximately 36 kDa in human heart lysates.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by ion exchange chromatography.
Buffer Solution
Tris buffered saline.
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Peptide with sequence C-RNRINDNQKVSKT, from the C-terminus of the protein
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt
P45379
Entrez Gene
TNNT2
GO Terms
GO:0003779 actin binding
GO:0005829 cytosol
GO:0005523 tropomyosin binding
GO:0005861 troponin complex
GO:0008016 regulation of heart contraction
GO:0030049 muscle filament sliding
GO:0030172 troponin C binding
GO:0031013 troponin I binding
GO:0032780 negative regulation of ATPase activity
GO:0032781 positive regulation of ATPase activity
GO:0051592 response to calcium ion
GO:0055010 ventricular cardiac muscle tissue morphogenesis

VPA00199

030615

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