Troponin T antibody

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Anti Troponin T (Cardiac) Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti Troponin T (Cardiac) Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Goat anti Human troponin T antibody (**VPA00199**) followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 21 second exposure. Arrow points to troponin T (molecular weight 36 kDa).

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Goat anti Troponin T (Cardiac)

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Troponin T

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Goat anti Human troponin T antibody recognizes troponin T also known as cardiomyopathy, dilated 1D (autosomal dominant), cardiomyopathy, hypertrophic 2 and troponin T cardiac muscle, .

The protein encoded by the TNNT2 gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in TNNT2 have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for TNNT2 undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined (provided by RefSeq, Jul 2008).

Goat anti Human troponin T antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti troponin T detects a band of approximately 36 kDa in human heart lysates.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by ion exchange chromatography.
Buffer Solution: Tris buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Immunogen: Peptide with sequence C-RNRINDNQKVSKT, from the C-terminus of the protein
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt: P45379
Entrez Gene: TNNT2
GO Terms: GO:0003779 actin binding; GO:0005829 cytosol; GO:0005523 tropomyosin binding; GO:0005861 troponin complex; GO:0008016 regulation of heart contraction; GO:0030049 muscle filament sliding; GO:0030172 troponin C binding; GO:0031013 troponin I binding; GO:0032780 negative regulation of ATPase activity; View More GO Terms; GO:0032781 positive regulation of ATPase activity; GO:0051592 response to calcium ion; GO:0055010 ventricular cardiac muscle tissue morphogenesis