TRIM5 Alpha antibody

Goat anti Human TRIM5 Alpha (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
TRIM5 Alpha
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1582
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.1 mg
List Price Your Price

Goat anti Human TRIM5 alpha antibody recognizes an epitope within the N-terminal (NT) region of human TRIM5alpha, otherwise known as RNF88 (RING finger protein 88), a cytoplasmic protein and member of the TRIM/RBCC family, known to play a vital role in species-specific early defence against retrovirus infection.

The association of TRIM5 with specific retroviral capsid sequences determines the effectiveness of the defence against infection. For example, in Old World monkeys, TRIM5alpha specifically associates with HIV type 1 (HIV-1), whereas in humans TRIM5alpha is not effective against HIV-1, but can inhibit strains of the murine leukaemia virus (MLV).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human TRIM5alpha (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence ASGILVNVKEEVTC corresponding to the N-Terminal region of TRIM5 (NP_149023.1; NP_149083.1; NP_149084.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/16000
Immunohistology - Paraffin 1 1.0ug/ml
Western Blotting 1.0 3.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human tonsil
Western Blotting
AHP1582 detects a band of approximately 37kDa in human brain cell lysates.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0

Further Reading

  1. Lee, K. & Kewalramani, V.N. (2004) In defense of the cell: TRIM5alpha interception of mammalian retroviruses.
    Proc Natl Acad Sci U S A. 101 (29): 10496-7.

Synonyms
RNF88
RRID
AB_2272282
UniProt
Q9C035
Entrez Gene
TRIM5

AHP1582

160610

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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