TRIM28 antibody
Rabbit anti TRIM28
TRIM28 antibody recognizes transcription intermediary factor 1-beta, also known as KAP1 , E3 SUMO-protein ligase TRIM28, KAP-1, KRIP-1, RING finger protein 96, TIF1-beta, or tripartite motif-containing protein 28.
TRIM28 Antibody with Control Lysate
TRIM28 antibody recognizes transcription intermediary factor 1-beta, also known as KAP1 , E3 SUMO-protein ligase TRIM28, KAP-1, KRIP-1, RING finger protein 96, TIF1-beta, nuclear corepressor KAP-1, or protein phosphatase 1.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00203 | WB | 100 µl | ||||
| VPA00203KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human TRIM28 antibody recognizes TRIM28, also known as KRAB-associated protein 1 (KAP1), transcription intermediary factor 1-beta, E3 SUMO-protein ligase, KRAB [Kruppel-associated box domain]-associated protein 1, KRAB-interacting protein 1, KRIP-1, RING finger protein 96, TIF1-beta, nuclear corepressor KAP-1, protein phosphatase 1, regulatory subunit 157, transcription intermediary factor 1-beta, transcriptional intermediary factor 1-beta, tripartite motif-containing 28 and tripartite motif-containing protein 28.
The TRIM28 protein mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. Localizes to the nucleus, TRIM28 is thought to associate with specific chromatin regions. This protein is a member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region (provided by RefSeq, Jul 2008).
Rabbit anti Human TRIM28 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The TRIM28 protein mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. Localizes to the nucleus, TRIM28 is thought to associate with specific chromatin regions. This protein is a member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region (provided by RefSeq, Jul 2008).
Rabbit anti Human TRIM28 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti TRIM28 detects a band of approximately 100 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide between 178-207 amino acids from the N-terminal region of human TRIM28
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q13263 Related reagents
- Entrez Gene
- TRIM28 Related reagents
- GO Terms
- GO:0001837 epithelial to mesenchymal transition
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003714 transcription corepressor activity
- GO:0008270 zinc ion binding
- GO:0043193 positive regulation of gene-specific transcription
- GO:0070087 chromo shadow domain binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of TRIM28 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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