TRIM28 antibody

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Anti TRIM28 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with TRIM28 antibody followed by detection with Goat anti Rabbit HRP conjugated(1/10,000,STAR208P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to TRIM28 (molecular weight 100 kDa)

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Rabbit anti TRIM28

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: TRIM28

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VPA00203 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human TRIM28 antibody recognizes TRIM28, also known as KRAB-associated protein 1 (KAP1), transcription intermediary factor 1-beta, E3 SUMO-protein ligase, KRAB [Kruppel-associated box domain]-associated protein 1, KRAB-interacting protein 1, KRIP-1, RING finger protein 96, TIF1-beta, nuclear corepressor KAP-1, protein phosphatase 1, regulatory subunit 157, transcription intermediary factor 1-beta, transcriptional intermediary factor 1-beta, tripartite motif-containing 28 and tripartite motif-containing protein 28.

The TRIM28 protein mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. Localizes to the nucleus, TRIM28 is thought to associate with specific chromatin regions. This protein is a member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region (provided by RefSeq, Jul 2008).

Rabbit anti Human TRIM28 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti TRIM28 detects a band of approximately 100 kDa in HeLa cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH conjugated synthetic peptide between 178-207 amino acids from the N-terminal region of human TRIM28
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q13263
Entrez Gene: TRIM28
GO Terms: GO:0001837 epithelial to mesenchymal transition; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003714 transcription corepressor activity; GO:0008270 zinc ion binding; GO:0043193 positive regulation of gene-specific transcription; GO:0070087 chromo shadow domain binding