TRIM28 antibody
Rabbit anti TRIM28
TRIM28 antibody recognizes transcription intermediary factor 1-beta, also known as KAP1 , E3 SUMO-protein ligase TRIM28, KAP-1, KRIP-1, RING finger protein 96, TIF1-beta, or tripartite motif-containing protein 28.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00203 | WB | 100 µl |
Rabbit anti Human TRIM28 antibody recognizes TRIM28, also known as KRAB-associated protein 1 (KAP1), transcription intermediary factor 1-beta, E3 SUMO-protein ligase, KRAB [Kruppel-associated box domain]-associated protein 1, KRAB-interacting protein 1, KRIP-1, RING finger protein 96, TIF1-beta, nuclear corepressor KAP-1, protein phosphatase 1, regulatory subunit 157, transcription intermediary factor 1-beta, transcriptional intermediary factor 1-beta, tripartite motif-containing 28 and tripartite motif-containing protein 28.
The TRIM28 protein mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. Localizes to the nucleus, TRIM28 is thought to associate with specific chromatin regions. This protein is a member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region (provided by RefSeq, Jul 2008).
Rabbit anti Human TRIM28 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The TRIM28 protein mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. Localizes to the nucleus, TRIM28 is thought to associate with specific chromatin regions. This protein is a member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region (provided by RefSeq, Jul 2008).
Rabbit anti Human TRIM28 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti TRIM28 detects a band of approximately 100 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide between 178-207 amino acids from the N-terminal region of human TRIM28
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q13263 Related reagents
- Entrez Gene
- TRIM28 Related reagents
- GO Terms
- GO:0001837 epithelial to mesenchymal transition
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003714 transcription corepressor activity
- GO:0008270 zinc ion binding
- GO:0043193 positive regulation of gene-specific transcription
- GO:0070087 chromo shadow domain binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of TRIM28 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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