TRIM28 antibody

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Rabbit anti TRIM28

TRIM28 antibody recognizes transcription intermediary factor 1-beta, also known as KAP1 , E3 SUMO-protein ligase TRIM28, KAP-1, KRIP-1, RING finger protein 96, TIF1-beta, or tripartite motif-containing protein 28.

TRIM28 Antibody with Control Lysate

TRIM28 antibody recognizes transcription intermediary factor 1-beta, also known as KAP1 , E3 SUMO-protein ligase TRIM28, KAP-1, KRIP-1, RING finger protein 96, TIF1-beta, nuclear corepressor KAP-1, or protein phosphatase 1.

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size
VPA00203	WB	100 µl
VPA00203KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Rabbit anti Human TRIM28 antibody recognizes TRIM28, also known as KRAB-associated protein 1 (KAP1), transcription intermediary factor 1-beta, E3 SUMO-protein ligase, KRAB [Kruppel-associated box domain]-associated protein 1, KRAB-interacting protein 1, KRIP-1, RING finger protein 96, TIF1-beta, nuclear corepressor KAP-1, protein phosphatase 1, regulatory subunit 157, transcription intermediary factor 1-beta, transcriptional intermediary factor 1-beta, tripartite motif-containing 28 and tripartite motif-containing protein 28.

The TRIM28 protein mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. Localizes to the nucleus, TRIM28 is thought to associate with specific chromatin regions. This protein is a member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region (provided by RefSeq, Jul 2008).

Rabbit anti Human TRIM28 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti TRIM28 detects a band of approximately 100 kDa in HeLa cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Rabbit polyclonal antibody purified by affinity chromatography

Preparation

20μl Rabbit polyclonal antibody purified by affinity chromatography

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

KLH conjugated synthetic peptide between 178-207 amino acids from the N-terminal region of human TRIM28

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Guarantee: As supplied, 12 months from date of despatch.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: Q13263 Related reagents
Entrez Gene: TRIM28 Related reagents
GO Terms: GO:0001837 epithelial to mesenchymal transition; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003714 transcription corepressor activity; GO:0008270 zinc ion binding; GO:0043193 positive regulation of gene-specific transcription; GO:0070087 chromo shadow domain binding
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of TRIM28 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB

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