TRIM28 antibody

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Rabbit anti TRIM28

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00203
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human TRIM28 antibody recognizes TRIM28, also known as KRAB-associated protein 1 (KAP1), transcription intermediary factor 1-beta, E3 SUMO-protein ligase, KRAB [Kruppel-associated box domain]-associated protein 1, KRAB-interacting protein 1, KRIP-1, RING finger protein 96, TIF1-beta, nuclear corepressor KAP-1, protein phosphatase 1, regulatory subunit 157, transcription intermediary factor 1-beta, transcriptional intermediary factor 1-beta, tripartite motif-containing 28 and tripartite motif-containing protein 28.

The TRIM28 protein mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. Localizes to the nucleus, TRIM28 is thought to associate with specific chromatin regions. This protein is a member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region (provided by RefSeq, Jul 2008).

Rabbit anti Human TRIM28 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti TRIM28 detects a band of approximately 100 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 178-207 amino acids from the N-terminal region of human TRIM28

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q13263 Related reagents
Entrez Gene
TRIM28 Related reagents
GO Terms
GO:0001837 epithelial to mesenchymal transition
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003714 transcription corepressor activity
GO:0008270 zinc ion binding
GO:0043193 positive regulation of gene-specific transcription
GO:0070087 chromo shadow domain binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of TRIM28 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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