TRIM25 antibody

Rabbit anti TRIM25

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
TRIM25

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VPA00619
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Rabbit anti Human TRIM25 antibody recognizes the E3 ubiquitin/ISG15 ligase TRIM25, also known as RING finger protein 147, estrogen-responsive finger protein, tripartite motif-containing protein 25, EFP or ubiquitin/ISG15-conjugating enzyme TRIM25.

The protein encoded by TRIM25 gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene (provided by RefSeq, Jul 2008).

Rabbit anti Human TRIM25 antibody detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti TRIM25 detects a band of approximately 72 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Immunogen
Synthetic peptide encompassing part of the middle region of human TRIM25
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
Q14258
Entrez Gene
TRIM25
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0009615 response to virus
GO:0005829 cytosol
GO:0004842 ubiquitin-protein ligase activity
GO:0005634 nucleus
GO:0008270 zinc ion binding
GO:0030054 cell junction
GO:0032480 negative regulation of type I interferon production
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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