TRIM25 antibody

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Anti TRIM25 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with TRIM25 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 76 second exposure. Arrow points to TRIM25 (molecular weight 72 kDa)

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Rabbit anti TRIM25

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: TRIM25

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VPA00619 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human TRIM25 antibody recognizes the E3 ubiquitin/ISG15 ligase TRIM25, also known as RING finger protein 147, estrogen-responsive finger protein, tripartite motif-containing protein 25, EFP or ubiquitin/ISG15-conjugating enzyme TRIM25.

The protein encoded by TRIM25 gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene (provided by RefSeq, Jul 2008).

Rabbit anti Human TRIM25 antibody detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Rabbit anti TRIM25 detects a band of approximately 72 kDa in HeLa cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide
2% Sucrose
Immunogen: Synthetic peptide encompassing part of the middle region of human TRIM25
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q14258
Entrez Gene: TRIM25
GO Terms: GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0009615 response to virus; GO:0005829 cytosol; GO:0004842 ubiquitin-protein ligase activity; GO:0005634 nucleus; GO:0008270 zinc ion binding; GO:0030054 cell junction; GO:0032480 negative regulation of type I interferon production; GO:0044419 interspecies interaction between organisms; View More GO Terms; GO:0045087 innate immune response