TRAF6 antibody

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Rabbit anti TRAF6

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP939 WB 0.1 mg
Rabbit anti Human TRAF6 antibody recognizes human TNF receptor-associated factor 6 (TRAF6).

TRAF6 is a signalling adaptor protein, which is common to both TLR and TNFR signalling pathways. TRAF6 has been shown to interact with interleukin-1 receptor-associated kinase 1 and 2 (IRAK1 and 2) and also to interact with TNFR family members CD40 and RANK.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human TRAF6 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide corresponding to fourteen amino acid residues within the C-Terminal region of human TRAF6.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9Y4K3 Related reagents
Entrez Gene
TRAF6 Related reagents
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0000187 activation of MAPK activity
GO:0000209 protein polyubiquitination
GO:0001503 ossification
GO:0002726 positive regulation of T cell cytokine production
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0004842 ubiquitin-protein ligase activity
GO:0005938 cell cortex
GO:0006916 anti-apoptosis
GO:0007250 activation of NF-kappaB-inducing kinase activity
GO:0007254 JNK cascade
GO:0008063 Toll signaling pathway
GO:0008270 zinc ion binding
GO:0008624 induction of apoptosis by extracellular signals
GO:0009898 internal side of plasma membrane
GO:0010008 endosome membrane
GO:0016564 transcription repressor activity
GO:0031293 membrane protein intracellular domain proteolysis
GO:0031435 mitogen-activated protein kinase kinase kinase binding
GO:0031965 nuclear membrane
GO:0032743 positive regulation of interleukin-2 production
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0035631 CD40 receptor complex
GO:0042826 histone deacetylase binding
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043422 protein kinase B binding
GO:0043507 positive regulation of JUN kinase activity
GO:0045087 innate immune response
GO:0045672 positive regulation of osteoclast differentiation
GO:0047485 protein N-terminus binding
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051403 stress-activated MAPK cascade
GO:0051865 protein autoubiquitination
GO:0070534 protein K63-linked ubiquitination
GO:0070555 response to interleukin-1
Regulatory
For research purposes only

Applications of TRAF6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1ug/ml 2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP939 detects a band of approximately 60kDa in PC-3 cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*