TRAF2 antibody
Goat anti TRAF2
TRAF2 antibody recognizes TNF receptor-associated factor 2, also known as E3 ubiquitin-protein ligase TRAF2 or tumor necrosis factor type 2 receptor-associated protein 3. The antibody has been extensively validated for western blotting using whole cell lysates.
TRAF2 Antibody with Control Lysate
TRAF2 antibody recognizes TNF receptor-associated factor 2, also known as E3 ubiquitin-protein ligase TRAF2 or tumor necrosis factor type 2 receptor-associated protein 3. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00051 | WB | 100 µl | ||||
| VPA00051KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human TRAF2 antibody recognizes human tumor necrosis factor (TNF) receptor associated factor 2 (TRAF2), a member of the TNF receptor associated factor (TRAF) protein family. TRAF2 is an adapter protein and signal transducer, which acts downstream of the TNF receptor. TRAF2 directly binds with TNF receptors and other TRAF proteins, and is essential for TNF alpha mediated activation of MAPK8/JNK and NF-kappaB. TRAF2 plays a role in apoptosis, through the formation of a heterodimeric complex with TRAF1, and interaction with the IAP family members cIAP1 and cIAP2, functioning as a mediator of the anti-apoptotic signals from TNF receptors.
Goat anti Human TRAF2 recognizes TRAF2 as a band of 46 kDa in a range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
Goat anti Human TRAF2 recognizes TRAF2 as a band of 46 kDa in a range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
Product Details
- Target Species
- Human
- Western Blotting
- Goat anti TRAF2 detects a band of approximately 46 kDa HEK293 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide sequence C-KMEAKNSYVRDD from the C-terminal region of TRAF2.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q12933 Related reagents
- Entrez Gene
- TRAF2 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0002726 positive regulation of T cell cytokine production
- GO:0005829 cytosol
- GO:0004842 ubiquitin-protein ligase activity
- GO:0004871 signal transducer activity
- GO:0005174 CD40 receptor binding
- GO:0006919 activation of caspase activity
- GO:0007250 activation of NF-kappaB-inducing kinase activity
- GO:0008270 zinc ion binding
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0008633 activation of pro-apoptotic gene products
- GO:0009898 internal side of plasma membrane
- GO:0032743 positive regulation of interleukin-2 production
- GO:0033209 tumor necrosis factor-mediated signaling pathway
- GO:0035631 CD40 receptor complex
- GO:0043507 positive regulation of JUN kinase activity
- GO:0043623 cellular protein complex assembly
- GO:0046625 sphingolipid binding
- GO:0050870 positive regulation of T cell activation
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051865 protein autoubiquitination
- GO:0070207 protein homotrimerization
- GO:0070534 protein K63-linked ubiquitination
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of TRAF2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
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