TRAF2 antibody

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Goat anti TRAF2

TRAF2 antibody recognizes TNF receptor-associated factor 2, also known as E3 ubiquitin-protein ligase TRAF2 or tumor necrosis factor type 2 receptor-associated protein 3. The antibody has been extensively validated for western blotting using whole cell lysates.

TRAF2 Antibody with Control Lysate

TRAF2 antibody recognizes TNF receptor-associated factor 2, also known as E3 ubiquitin-protein ligase TRAF2 or tumor necrosis factor type 2 receptor-associated protein 3. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00051 WB 100 µl
VPA00051KT WB 2 Westerns
With Lysate 400µg
Goat anti Human TRAF2 antibody recognizes human tumor necrosis factor (TNF) receptor associated factor 2 (TRAF2), a member of the TNF receptor associated factor (TRAF) protein family. TRAF2 is an adapter protein and signal transducer, which acts downstream of the TNF receptor. TRAF2 directly binds with TNF receptors and other TRAF proteins, and is essential for TNF alpha mediated activation of MAPK8/JNK and NF-kappaB. TRAF2 plays a role in apoptosis, through the formation of a heterodimeric complex with TRAF1, and interaction with the IAP family members cIAP1 and cIAP2, functioning as a mediator of the anti-apoptotic signals from TNF receptors.

Goat anti Human TRAF2 recognizes TRAF2 as a band of 46 kDa in a range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Goat anti TRAF2 detects a band of approximately 46 kDa HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-KMEAKNSYVRDD from the C-terminal region of TRAF2.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q12933 Related reagents
Entrez Gene
TRAF2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0002726 positive regulation of T cell cytokine production
GO:0005829 cytosol
GO:0004842 ubiquitin-protein ligase activity
GO:0004871 signal transducer activity
GO:0005174 CD40 receptor binding
GO:0006919 activation of caspase activity
GO:0007250 activation of NF-kappaB-inducing kinase activity
GO:0008270 zinc ion binding
GO:0008624 induction of apoptosis by extracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0009898 internal side of plasma membrane
GO:0032743 positive regulation of interleukin-2 production
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0035631 CD40 receptor complex
GO:0043507 positive regulation of JUN kinase activity
GO:0043623 cellular protein complex assembly
GO:0046625 sphingolipid binding
GO:0050870 positive regulation of T cell activation
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051865 protein autoubiquitination
GO:0070207 protein homotrimerization
GO:0070534 protein K63-linked ubiquitination
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of TRAF2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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