Rabbit anti Human Toso antibody recognizes the C-terminal (CT) region of human Toso, also known as Fas Apoptotic Inhibitory Molecule 3 (FAIM3). Toso is expressed by lymphoid and haematopoietic cells and is up-regulated by T-cell activation. Toso expressing cells have increased resistance to Fas-, FADD-, and TNFalpha-induced apoptosis. Toso is membrane bound, and is thought to directly bind to FADD, disrupting DISC formation and thus inhibiting caspase-8 activation.
Toso is a potential proto-oncogene, and could be a therapeutic target for use in the treatment of autoimmune diseases. Despite its predicted molecular mass of ~30-44 kDa, Toso often migrates at ~60 kDa in SDS PAGE.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human Toso were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- A 13 amino acid peptide from near the carboxy terminus of human Toso.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
cellular defense response
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of TOSO antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1183 detects a band of approximately 43kDa in human lung tissue lysate.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Hitoshi, Y. et al. (1998) Toso, a cell surface, specific regulator of Fas-induced apoptosis in T cells.
Immunity. 8 (4): 461-71.
Song, Y. & Jacob, C.O. (2005) The mouse cell surface protein TOSO regulates Fas/Fas ligand-induced apoptosis through its binding to Fas-associated death domain.
J Biol Chem. 280 (10): 9618-26.
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