TOSO antibody

Rabbit anti Human TOSO (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
TOSO
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1183
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Human Toso antibody recognizes the C-terminal (CT) region of human Toso, also known as Fas Apoptotic Inhibitory Molecule 3 (FAIM3). Toso is expressed by lymphoid and haematopoietic cells and is up-regulated by T-cell activation. Toso expressing cells have increased resistance to Fas-, FADD-, and TNFalpha-induced apoptosis. Toso is membrane bound, and is thought to directly bind to FADD, disrupting DISC formation and thus inhibiting caspase-8 activation.

Toso is a potential proto-oncogene, and could be a therapeutic target for use in the treatment of autoimmune diseases. Despite its predicted molecular mass of ~30-44 kDa, Toso often migrates at ~60 kDa in SDS PAGE.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human Toso were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
A 13 amino acid peptide from near the carboxy terminus of human Toso.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1183 detects a band of approximately 43kDa in human lung tissue lysate.

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Further Reading

  1. Hitoshi, Y. et al. (1998) Toso, a cell surface, specific regulator of Fas-induced apoptosis in T cells.
    Immunity. 8 (4): 461-71.
  2. Song, Y. & Jacob, C.O. (2005) The mouse cell surface protein TOSO regulates Fas/Fas ligand-induced apoptosis through its binding to Fas-associated death domain.
    J Biol Chem. 280 (10): 9618-26.

Synonyms
FAIM3
RRID
AB_2101067
UniProt
O60667
Entrez Gene
FAIM3
GO Terms
GO:0016021 integral to membrane
GO:0006916 anti-apoptosis
GO:0006968 cellular defense response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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