TMS1 antibody

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Goat anti TMS1

Goat anti human TMS1 antibody recognizes apoptosis-associated speck-like protein containing a CARD, also known as PYCARD, PYD and CARD domain containing, ASC, or target of methylation-induced silencing 1. This antibody has been extensively validated for western blotting using whole cell lysates.

TMS1 Antibody with Control Lysate

Goat anti human TMS1 antibody recognizes apoptosis-associated speck-like protein containing a CARD, also known as PYCARD, PYD and CARD domain containing, ASC, caspase recruitment domain-containing protein 5, CARD5 or target of methylation-induced silencing 1.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00421 WB 100 µl
VPA00421KT WB 2 Westerns
With Lysate 400µg
Goat anti Human TMS1 antibody recognizes target of methylation-induced silencing 1 (TMS1), also known as PYCARD, caspase recruitment domain-containing protein 5 and CARD5.

TMS1 is an adaptor protein composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for PYCARD (provided by RefSeq, Jul 2008).

Goat anti Human TMS1 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti TMS1 detects a band of approximately 22 kDa in A549 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Peptide sequence C-DLTDKLVSYYLES
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg A549 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9ULZ3 Related reagents
Entrez Gene
PYCARD Related reagents
GO Terms
GO:0004197 cysteine-type endopeptidase activity
GO:0006508 proteolysis
GO:0006917 induction of apoptosis
GO:0006919 activation of caspase activity
GO:0008385 IkappaB kinase complex
GO:0008656 caspase activator activity
GO:0032090 Pyrin domain binding
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0042803 protein homodimerization activity
GO:0050718 positive regulation of interleukin-1 beta secretion
GO:0051092 positive regulation of NF-kappaB transcription factor activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of TMS1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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