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TL1A antibody

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Anti TL1A (N-Terminal) Antibody gallery image 1
Western blot analysis of whole cell lysate from PC-3 human prostate cancer cell line probed with Rabbit anti Human TL1A (AHP1661).

Rabbit anti TL1A (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1661
Datasheet
WB
SDS
0.1 mg loader

Rabbit anti TL1A (N-Terminal) antibody recognizes the amino Terminal of human TL1A, also known as tumour necrosis factor ligand superfamily member 15 (TNFSF15). TL1A acts as a co-stimulator of IFN-gamma secretion through binding to DR3 (death domain receptor 3 or TRAMP) and may play an important role in Th1-mediated diseases such as Crohn’s disease. In cells expressing DR3, TL1A induces NF-kappa-B activation and apoptosis. Decoy receptor 3 (DcR3) is also a receptor for TL1A. DcR3 can apparently neutralise the effect of TL1A, by inhibiting the TL1A-DR3 interaction, thereby inhibiting apoptosis. TL1A is specifically expressed on endothelial cells and is detected on monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to TL1A (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by immunoaffinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to 14 amino acids near the amino terminus of human TL1A.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O95150
Entrez Gene
TNFSF15
GO Terms
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0006919 activation of caspase activity
GO:0006955 immune response
GO:0007250 activation of NF-kappaB-inducing kinase activity
GO:0042107 cytokine metabolic process
Regulatory
For research purposes only

Applications of TL1A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1661 detects a band of approximately 27kDa in PC-3 cell lysates
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Wen, L. et al. (2003) TL1A-induced NF-kappaB activation and c-IAP2 production prevent DR3-mediated apoptosis in TF-1 cells.
    J Biol Chem. 278 (40): 39251-8.
  2. Bamias, G. et al. (2003) Expression, localization, and functional activity of TL1A, a novel Th1-polarizing cytokine in inflammatory bowel disease.
    J Immunol. 171 (9): 4868-74.
  3. Yang, C.R. et al. (2004) Soluble decoy receptor 3 induces angiogenesis by neutralization of TL1A, a cytokine belonging to tumor necrosis factor superfamily and exhibiting angiostatic action.
    Cancer Res. 64 (3): 1122-9.

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Request a different product with the TL1A specificity