Tissue Transglutaminase antibody

Rabbit anti Tissue Transglutaminase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Tissue Transglutaminase

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AHP2532
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SDS Safety Datasheet SDS
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Rabbit anti tissue transglutaminase antibody recognizes tissue transglutaminase, also known as protein-glutamine gamma-glutamyltransferase 2. Tissue transglutaminase belongs to the transglutaminase family of proteins and is an enzyme which catalyzes protein cross-linking and addition of polyamines to proteins.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to tissue transglutaminase was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant human tissue transglutaminase
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/100 1/200
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti tissue transglutaminase antibody detects a band of approximately 90 kDa in cell lysates under reducing conditions

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UniProt
P21980
Entrez Gene
TGM2
GO Terms
GO:0003810 protein-glutamine gamma-glutamyltransferase activity
GO:0046872 metal ion binding
GO:0008415 acyltransferase activity
GO:0043277 apoptotic cell clearance
GO:0045785 positive regulation of cell adhesion