TIMP-2 antibody
Sheep anti Human TIMP-2 antibody (9013-2609) used to assess levels of TIMP-2 in murine melanoma cell lysates by western blotting.
Image caption:
Effects of DNE3 on the protein levels of proteases and their endogenous inhibitors.
Cells were treated with DNE3 for 24 hours and then cell lysates were subjected to Western blot to analyze the expression of MMP-2 (c) and TIMP-2 (d). Results were statistically evaluated by using one-way ANOVA with post hoc Dunnett's test (*P <.05; ***P <.001).
From: Ho ML, Hsieh YS, Chen JY, Chen KS, Chen JJ, Kuo WH, Lin SJ, Chen PN.
Antimetastatic Potentials of Dioscorea nipponica on Melanoma In Vitro and In Vivo.
Evid Based Complement Alternat Med. 2011;2011:507920.
doi: 10.1155/2011/507920.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB ResetSheep anti Human TIMP-2 (Loop 6)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- TIMP-2
- Region
- (LOOP 6)
Antibody Quality and Performance Guarantee
| Sheep anti Human TIMP-2 antibody recognizes human TIMP-2, a tissue inhibitor that complexes with and irreversibly inactivates various metalloproteinases. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to the loop 6 region of the native molecule conjugated to Keyhole Limpet Haemocyanin.
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
||
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
 |
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Rabbit anti Sheep IgG (H/L):Biotin | ||||||
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Donkey anti Sheep/Goat IgG:DyLight®488 | ||||||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Donkey anti Sheep/Goat IgG:HRP | ||||||
References for TIMP-2 antibody
-
Chu, S.C. et al. (2004) Silibinin inhibits the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase-2.
Mol Carcinog. 40 (3): 143-9. -
Chen, P.N. et al. (2006) Mulberry anthocyanins, cyanidin 3-rutinoside and cyanidin 3-glucoside, exhibited an inhibitory effect on the migration and invasion of a human lung cancer cell line.
Cancer Lett. 235 (2): 248-59. -
Ho, M.L. et al. (2011) Antimetastatic Potentials of Dioscorea nipponica on Melanoma In Vitro and In Vivo
Evidence-Based Complementary and Alternative Medicine 2011 Article ID 507920 -
Jaworski, D.M. et al. (2011) Sexually dimorphic diet-induced insulin resistance in obese tissue inhibitor of metalloproteinase-2 (TIMP-2)-deficient mice.
Endocrinology. 152 (4): 1300-13.
- RRID
- AB_620423
- UniProt
- P16035
- Entrez Gene
- TIMP2
- GO Terms
- GO:0046872 metal ion binding
- GO:0008191 metalloendopeptidase inhibitor activity
9013-2609
151029
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with TIMP-2 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up