TIMP-1 antibody
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P ResetRabbit anti Human TIMP-1
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- TIMP-1
Antibody Quality and Performance Guarantee
| Rabbit anti Human TIMP-1 antibody recognizes human TIMP-1 (Tissue Inhibitor of Metalloproteinase -1), a secreted glycoprotein and member of the protease inhibitor I35 (TIMP) family, which is expressed by a wide range of cells, including microvascular endothelial cells, smooth muscle cells, mesangial cells, and some fibroblasts. TIMP-1 acts as a natural extracellular inhibitor of MMPs (matrix metalloproteinases), a group of peptidases involved in degradation of the extracellular matrix, and is also involved in erythropoiesis, through the simulation of the proliferation/differentiation of erythroid progenitors. A correlation between localized concentrations of TIMP-1 and TIMP-3, may determine cell fate, and that increased expression of TIMP-1, may act as an inhibitor/suppressor of invasive cell growth (Sternlicht and Werb 2001) |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to human TIMP-1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human TIMP-1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
||
| Immunohistology - Paraffin 1 | |
||
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1 This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This purified human TIMP-1 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human TIMP-1 antibody (AHP2187B) as the detection reagent.
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- RRID
- AB_10842664
- UniProt
- P01033
- Entrez Gene
- TIMP1
- GO Terms
- GO:0005515 protein binding
- GO:0002576 platelet degranulation
- GO:0046872 metal ion binding
- GO:0005578 proteinaceous extracellular matrix
- GO:0008191 metalloendopeptidase inhibitor activity
- GO:0008284 positive regulation of cell proliferation
- GO:0030168 platelet activation
- GO:0031093 platelet alpha granule lumen
- GO:0043249 erythrocyte maturation
- View More GO Terms
- GO:0051045 negative regulation of membrane protein ectodomain proteolysis
AHP2187
140715
200115
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