TIMP-1 antibody

Rabbit anti Human TIMP-1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
TIMP-1

Product Code Applications Pack Size List Price Your Price Qty
9013-1108
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Human TIMP-1 antibody recognizes human Tissue inhibitor of metalloproteinases 1, also known as TIMP-1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity or Fibroblast collagenase inhibitor. TIMP-1 is a 207 amino acid, ~23 kDa secreted glycoprotein containing a 23 amino acid signaling peptide and a single NTR domain. TIMP-1 is a metalloproteinase inhibitor that functions by formation of complexes with target metalloproteinases, binding to their catalytic cofactor leading to irreversible inactivation of the target metalloproteinase. TIMP-1 also functions as a growth factor and activates signalling cascades via CD63 and CD29 (Uniprot: P01033). In humans TIMP-1 also functions to promote growth and differentiation of erythroid progenitors (Gasson et al. 1985).

Rabbit anti Human TIMP-1 antibody recognizes both native and denatured TIMP-1, as well as TIMP-1 in and out of enzyme/inhibitor complex. This antibody is not known to be neutralizing.TIMP-1 is expressed by a wide range of cells including microvascular endothelial cells, smooth muscle cells, mesangial cells and some fibroblasts.

Rabbit anti Humn TIMP-1 antibody has been used successfully for the localization of TIMP-1 in a rabbit model of hypertrophic scarring by immunohistochemistry on formalin fixed, paraffin embedded sections (Tark et al. 2015).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Horse
Rabbit
Bovine
Ferret
Rat
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
Human tissue inhibitor of metalloproteinase 1 (TIMP-1).
Approx. Protein Concentrations
1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 2.5 µg/ml 10 µg/ml
Immunohistology - Paraffin 1 µg/ml 5 µg/ml
Western Blotting 5 µg/ml 10 µg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for TIMP-1 antibody

  1. O'Kane, C.M. et al. (2010) STAT3, p38 MAPK, and NF-{kappa}B Drive Unopposed Monocyte-DependentFibroblast MMP-1 Secretion in Tuberculosis.
    Am J Respir Cell Mol Biol. 43: 465-74.
  2. Tark, K.C. et al. (2015) Effects of ginsenoside Rb1 on hypertrophic scar remodeling in rabbit model.
    Eur J Pharmacol. 750C: 151-59.

RRID
AB_2204551
UniProt
P01033
Entrez Gene
TIMP1
GO Terms
GO:0005515 protein binding
GO:0002576 platelet degranulation
GO:0046872 metal ion binding
GO:0005578 proteinaceous extracellular matrix
GO:0008191 metalloendopeptidase inhibitor activity
GO:0008284 positive regulation of cell proliferation
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0043249 erythrocyte maturation
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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