Thymidylate Synthase antibody
Sheep anti Human thymidylate synthase antibody recognizes thymidylate synthase, also known as TSase
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of thymidylate synthase and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase (provided by RefSeq, Jul 2008).
Sheep anti Human thymidylate synthase antibody detects a band of 31 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of thymidylate synthase and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase (provided by RefSeq, Jul 2008).
Sheep anti Human thymidylate synthase antibody detects a band of 31 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti thymidylate synthase detects a band of approximately 31 kDa in Jurkat cell lysates.
- Product Form
- Ig fraction - liquid
- Preparation
- Sheep polyclonal Ig fraction prepared prepared by ammonium sulfate precipitation
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant human thymidylate synthase
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P04818
- Entrez Gene
- TYMS
- GO Terms
- GO:0006260 DNA replication
- GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
- GO:0005829 cytosol
- GO:0004799 thymidylate synthase activity
- GO:0006206 pyrimidine base metabolic process
- GO:0006281 DNA repair
- GO:0046683 response to organophosphorus
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0046134 pyrimidine nucleoside biosynthetic process
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Thymidylate Synthase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
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