Thymidylate Synthase antibody

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Anti Thymidylate Synthase Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Sheep anti Human thymidylate synthase antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 78 second exposure. Arrow points to thymidylate synthase (molecular weight 31 kDa).

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Sheep anti Thymidylate Synthase

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Thymidylate Synthase

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Sheep anti Human thymidylate synthase antibody recognizes thymidylate synthase, also known as TSase

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of thymidylate synthase and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase (provided by RefSeq, Jul 2008).

Sheep anti Human thymidylate synthase antibody detects a band of 31 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti thymidylate synthase detects a band of approximately 31 kDa in Jurkat cell lysates.
Product Form: Ig fraction - liquid
Preparation: Sheep polyclonal Ig fraction prepared prepared by ammonium sulfate precipitation.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Recombinant human thymidylate synthase
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt: P04818
Entrez Gene: TYMS
GO Terms: GO:0006260 DNA replication; GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle; GO:0005829 cytosol; GO:0004799 thymidylate synthase activity; GO:0006206 pyrimidine base metabolic process; GO:0006281 DNA repair; GO:0046683 response to organophosphorus; GO:0048015 phosphatidylinositol-mediated signaling; GO:0046134 pyrimidine nucleoside biosynthetic process; View More GO Terms