Thymidylate Synthase antibody

Sheep anti Thymidylate Synthase

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Thymidylate Synthase

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VPA00186
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Sheep anti Human thymidylate synthase antibody recognizes thymidylate synthase, also known as TSase

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of thymidylate synthase and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase (provided by RefSeq, Jul 2008).

Sheep anti Human thymidylate synthase antibody detects a band of 31 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti thymidylate synthase detects a band of approximately 31 kDa in Jurkat cell lysates.
Product Form
Ig fraction - liquid
Preparation
Sheep polyclonal Ig fraction prepared prepared by ammonium sulfate precipitation.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant human thymidylate synthase
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P04818
Entrez Gene
TYMS
GO Terms
GO:0006260 DNA replication
GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
GO:0005829 cytosol
GO:0004799 thymidylate synthase activity
GO:0006206 pyrimidine base metabolic process
GO:0006281 DNA repair
GO:0046683 response to organophosphorus
GO:0048015 phosphatidylinositol-mediated signaling
GO:0046134 pyrimidine nucleoside biosynthetic process