THRAP3 antibody
Rabbit anti THRAP3
Rabbit anti human THRAP3 antibody recognizes thyroid hormone receptor-associated protein 3, also known as thyroid hormone receptor-associated protein complex 150 kDa component. This antibody has been extensively validated for western blotting using whole cell lysates.
THRAP3 Antibody with Control Lysate
Rabbit anti human THRAP3 antibody recognizes thyroid hormone receptor-associated protein 3, also known as thyroid hormone receptor-associated protein complex 150 kDa component. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00342 | WB | 100 µl | ||||
| VPA00342KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human THRAP3 antibody recognizes THRAP3, also known as thyroid hormone receptor-associated protein complex 150 kDa component and thyroid hormone receptor-associated protein 3.
Rabbit anti Human THRAP3 antibody detects a band of 150 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Rabbit anti Human THRAP3 antibody detects a band of 150 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti THRAP3 detects a band of approximately 150 kDa in Jurkat cell lysate
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 927-955 of human THRAP3
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q9Y2W1 Related reagents
- Entrez Gene
- THRAP3 Related reagents
- GO Terms
- GO:0004872 receptor activity
- GO:0005524 ATP binding
- GO:0006367 transcription initiation from RNA polymerase II promoter
- GO:0016455 RNA polymerase II transcription mediator activity
- GO:0016563 transcription activator activity
- GO:0016592 mediator complex
- GO:0046966 thyroid hormone receptor binding
- GO:0030374 ligand-dependent nuclear receptor transcription coactivator activity
- GO:0030521 androgen receptor signaling pathway
- GO:0042809 vitamin D receptor binding
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of THRAP3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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