THRAP3 antibody

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Rabbit anti THRAP3

Rabbit anti human THRAP3 antibody recognizes thyroid hormone receptor-associated protein 3, also known as thyroid hormone receptor-associated protein complex 150 kDa component. This antibody has been extensively validated for western blotting using whole cell lysates.

THRAP3 Antibody with Control Lysate

Rabbit anti human THRAP3 antibody recognizes thyroid hormone receptor-associated protein 3, also known as thyroid hormone receptor-associated protein complex 150 kDa component. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00342 WB 100 µl
VPA00342KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human THRAP3 antibody recognizes THRAP3, also known as thyroid hormone receptor-associated protein complex 150 kDa component and thyroid hormone receptor-associated protein 3.

Rabbit anti Human THRAP3 antibody detects a band of 150 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti THRAP3 detects a band of approximately 150 kDa in Jurkat cell lysate
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 927-955 of human THRAP3
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9Y2W1 Related reagents
Entrez Gene
THRAP3 Related reagents
GO Terms
GO:0004872 receptor activity
GO:0005524 ATP binding
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0016455 RNA polymerase II transcription mediator activity
GO:0016563 transcription activator activity
GO:0016592 mediator complex
GO:0046966 thyroid hormone receptor binding
GO:0030374 ligand-dependent nuclear receptor transcription coactivator activity
GO:0030521 androgen receptor signaling pathway
GO:0042809 vitamin D receptor binding
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of THRAP3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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