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Thioredoxin antibody

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Western Blot Validated
Anti Thioredoxin Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with thioredoxin antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 18 second exposure. Arrow points to thioredoxin (molecular weight 12 kDa)

Rabbit anti Thioredoxin

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00739
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human thioredoxin antibody recognizes thioredoxin also known as ADF, ATL-derived factor, SASP delta 3, surface-associated sulphydryl protein or TXN delta 3.

The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene (provided by RefSeq, Oct 2011).

Rabbit anti Human thioredoxin antibody detects a band of 12 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti thioredoxin antibody recognizes a band of approximately 12 kDa in HepG2 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on Protein A
Buffer Solution
HEPES buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
E. coli-derived recombinant protein of human thioredoxin

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P10599
Entrez Gene
TXN
GO Terms
GO:0005515 protein binding
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0007165 signal transduction
GO:0005654 nucleoplasm
GO:0022900 electron transport chain
GO:0006350 transcription
GO:0006810 transport
GO:0006928 cellular component movement
GO:0007267 cell-cell signaling
GO:0008283 cell proliferation
GO:0009314 response to radiation
GO:0015949 nucleobase, nucleoside and nucleotide interconversion
GO:0033158 regulation of protein import into nucleus, translocation
GO:0043388 positive regulation of DNA binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Thioredoxin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
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