TGF Alpha antibody

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Sheep anti Human TGF Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
9129-7995 E 0.5 mg
Sheep anti Human TGF alpha antibody recognizes TGF-alpha (transforming growth factor alpha) from human, rat and bovine tissues and fluids. TGF-alpha is a 50 amino acid EGF-related polypeptide expressed in a range of tissues, including skin, pancreas, liver, duodenum, salivary glands, urinary and respiratory systems, and the developing and mature central nervous system (CNS)

TGF-alpha is produced by monocytes, keratinocytes and neuronal cells and stimulates the proliferation of a range of epidermal and epithelial cells, through binding to the epidermal growth factor receptor (EGFR). TGF-alpha is mitogenic for multipotent neural progenitors and is involved in astrogliosis.

This antibody does not react with EGF or TGF-beta.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified Ig - liquid
Preparation
Purified Ig prepared by ion exchange chromatography
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Mature human recombinant TGF-alpha conjugated to KLH.
Approx. Protein Concentrations
IgG concentration 3.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01135 Related reagents
Entrez Gene
TGFA Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000187 activation of MAPK activity
GO:0016021 integral to membrane
GO:0004708 MAP kinase kinase activity
GO:0004871 signal transducer activity
GO:0005154 epidermal growth factor receptor binding
GO:0045741 positive regulation of epidermal growth factor receptor activity
GO:0005615 extracellular space
GO:0008083 growth factor activity
GO:0008283 cell proliferation
GO:0009986 cell surface
GO:0045840 positive regulation of mitosis
GO:0050679 positive regulation of epithelial cell proliferation
GO:0051781 positive regulation of cell division
Regulatory
For research purposes only

Applications of TGF Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Product Specific References

References for TGF Alpha antibody

  1. Ju, W. et al. (1991) Tumorigenic transformation of NIH 3T3 cells by the autocrine synthesis of transforming growth factor alpha.
    New Biol. 3: 380-388.