Tfam antibody
Rabbit anti Tfam
Rabbit anti-human TFAM antibody recognizes transcription factor A, mitochondrial, also known as mitochondrial transcription factor 1 or transcription factor 6-like 3. This antibody has been extensively validated for western blotting using whole cell lysates.
Tfam Antibody with Control Lysate
Rabbit anti-human TFAM antibody recognizes transcription factor A, mitochondrial, also known as mitochondrial transcription factor 1 or transcription factor 6-like 3. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00464 | WB | 100 µl | ||||
| VPA00464KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human TFAM antibody recognizes TFAM, mitochondrial, also known as mitochondrial transcription factor 1, mitochondrial transcription factor A, transcription factor 6, or transcription factor 6-like 2.
The TFAM gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in TFAM are associated with Alzheimer's and Parkinson's diseases (Belin et al. 2007). There are pseudogenes for TFAM on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants (provided by RefSeq, Aug 2012).
Rabbit anti Human TFAM antibody detects a band of 29 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The TFAM gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in TFAM are associated with Alzheimer's and Parkinson's diseases (Belin et al. 2007). There are pseudogenes for TFAM on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants (provided by RefSeq, Aug 2012).
Rabbit anti Human TFAM antibody detects a band of 29 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide directed towards the N terminal region of human TFAM
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q00059 Related reagents
- Entrez Gene
- TFAM Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005634 nucleus
- GO:0006261 DNA-dependent DNA replication
- GO:0006356 regulation of transcription from RNA polymerase I promoter
- GO:0006391 transcription initiation from mitochondrial promoter
- GO:0042645 mitochondrial nucleoid
- GO:0045893 positive regulation of transcription, DNA-dependent
- GO:0070363 mitochondrial light strand promoter sense binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Tfam antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Anti TFAM detects a band of approximately 29 kDa in Jurkat cell lysates
- Western Blotting
- Anti TFAM detects a band of approximately 29 kDa in Jurkat cell lysates
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
