Sheep anti Human Tamm Horsfall Glycoprotein antibody
recognizes human Tamm Horsfall Protein (THP), also known as uromodulin (Pennica et al. 1987
). THP was originally isolated from normal human urine (Tamm et al. 1952
). THP is a 616 amino acid ~85 kDa glycoprotein synthesized by the kidney and is the most abundant urinary protein in healthy individuals (Pennica et al. 1987
). THP forms the matrix of all urinary casts either alone (hyaline casts) or in association with cellular fragments and filtered proteins (granular casts), casts are indicative of a number of pathological states including glomerulonephritis and vasculitis (Chambers et al. 1986
The specificity of sheep anti human Tamm Horsfall glycoprotein antibody has been confirmed by double diffusion against tamm horsfall glycoprotein and an independent known antibody against THP.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- IgG fraction - liquid
- IgG fraction prepared by anion exchange chromatography
- Buffer Solution
- Glycine buffered saline
- Preservative Stabilisers
|0.09%||Sodium Azide (NaN3)|
- Purified human uromucodulin prepared from urine.
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
- Guaranteed until date of expiry. Please see product label.
- Entrez Gene
- GO Terms
calcium ion binding
cellular defense response
negative regulation of cell proliferation
anchored to membrane
apical plasma membrane
extrinsic to membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Tamm Horsfall Glycoprotein antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Application Based External Images
Product Specific References
References for Tamm Horsfall Glycoprotein antibody
El-Achkar, T.M. et al. (2011) Tamm-Horsfall protein-deficient thick ascending limbs promote injury to neighboring S3 segments in an MIP-2-dependent mechanism.
Am J Physiol Renal Physiol. 300 (4): F999-1007.
Hao, S. et al. (2011) Differential regulation of NFAT5 by NKCC2 isoforms in medullary thick ascending limb (mTAL) cells.
Am J Physiol Renal Physiol. 300: F966-75.
Vekaria, R.M. et al. (2006) Immunolocalization of ectonucleotidases along the rat nephron.
Am J Physiol Renal Physiol. 290 (2): F550-60.
El-Achkar, T.M. et al. (2013) Tamm-Horsfall protein translocates to the basolateral domain of thick ascending limbs, interstitium, and circulation during recovery from acute kidney injury.
Am J Physiol Renal Physiol. 304 (8): F1066-75.
El-Achkar, T.M. et al. (2008) Tamm-Horsfall protein protects the kidney from ischemic injury by decreasing inflammation and altering TLR4 expression.
Am J Physiol Renal Physiol. 295 (2): F534-44.
Nevo, N. et al. (2010) Renal phenotype of the cystinosis mouse model is dependent upon genetic background.
Nephrol Dial Transplant. 25: 1059-66.
Venables, G. et al. (2011) Ghrelin receptors are expressed by distal tubules of the mouse kidney.
Cell Tissue Res. 346: 135-9.
Rice, J.C. et al. (2002) Monocyte chemoattractant protein-1 expression correlates with monocyte infiltration in the post-ischemic kidney.
Ren Fail. 24: 703-23.
Liu, S. et al. (2012) Autophagy plays a critical role in kidney tubule maintenance, aging and ischemia-reperfusion injury.
Autophagy. 8: 826-37.
Saito, S. et al. (2011) Analysis of glial cell line-derived neurotrophic factor-inducible zinc finger protein 1 expression in human diseased kidney.
Hum Pathol. 42: 848-58.
Ferrè, S. et al. (2014) Mutations in PCBD1 cause hypomagnesemia and renal magnesium wasting.
J Am Soc Nephrol. 25 (3): 574-86.
Maeda-Hori, M. et al. (2014) Plasma CD147 reflects histological features in patients with lupus nephritis.
Lupus. 23: 342-52.
Maruyama, H. et al. (2018) Medullary thick ascending limb impairment in the GlatmTg(CAG-A4GALT) Fabry model mice.
FASEB J. 32 (8): 4544-59.
Choi, H.I. et al. (2019) Peroxiredoxin V (PrdxV) negatively regulates EGFR/Stat3-mediated fibrogenesis via a Cys48-dependent interaction between PrdxV and Stat3.
Sci Rep. 9 (1): 8751.
Maydan, O. et al. (2018) Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy.
Physiol Rep. 6 (6): e13654.
Heitmeier, M. et al. (2014) The role of tumor necrosis factor alpha in regulating the expression of Tamm-Horsfall Protein (uromodulin) in thick ascending limbs during kidney injury.
Am J Nephrol. 40 (5): 458-67.