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Tamm Horsfall Glycoprotein antibody

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Anti Human Tamm Horsfall Glycoprotein Antibody thumbnail image 1 Anti Human Tamm Horsfall Glycoprotein Antibody thumbnail image 2 Anti Human Tamm Horsfall Glycoprotein Antibody thumbnail image 3 Anti Human Tamm Horsfall Glycoprotein Antibody thumbnail image 4 Anti Human Tamm Horsfall Glycoprotein Antibody thumbnail image 5 Anti Human Tamm Horsfall Glycoprotein Antibody thumbnail image 6 Anti Human Tamm Horsfall Glycoprotein Antibody thumbnail image 7 Anti Human Tamm Horsfall Glycoprotein Antibody thumbnail image 8 Anti Human Tamm Horsfall Glycoprotein Antibody thumbnail image 9
Anti Human Tamm Horsfall Glycoprotein Antibody gallery image 1
Published customer image:
Sheep anti Human Tamm Horsfall Glycoprotein antibody (8595-0054) used to label THP expressing cells in mouse kidney tissue sections by immunofluorescence.
Image caption:
Comparison of PrdxV expression between PrdxVwt and PrdxVsi mouse kidney. (c-f) Tubule specific expression of PrdxV (Red) were assessed by tubule markers (Green), AQP1, THP, Calbindin D28K, and AQP2, using immunofluorescence. Nucleus was stained with DAPI. Image was magnified at x400.

From: Choi HI, Kim DH, Park JS, Kim IJ, Kim CS, Bae EH, Ma SK, Lee TH, Kim SW.
Peroxiredoxin V (PrdxV) negatively regulates EGFR/Stat3-mediated fibrogenesis via a Cys48-dependent interaction between PrdxV and Stat3.
Sci Rep. 2019 Jun19;9(1):8751.
doi: 10.1038/s41598-019-45347-0.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Tamm Horsfall Glycoprotein Antibody gallery image 2
Published customer image:
Sheep anti Human Tamm Horsfall Glycoprotein antibody (8595-0054) used to label THP expressing cells in mouse kidney tissue sections by immunofluorescence. Detail of previous image, panel d only.
Image caption:
Comparison of PrdxV expression between PrdxVwt and PrdxVsi mouse kidney. (c-f) Tubule specific expression of PrdxV (Red) were assessed by tubule marker (Green), THP using immunofluorescence. Nucleus was stained with DAPI. Image was magnified at x400.

From: Choi HI, Kim DH, Park JS, Kim IJ, Kim CS, Bae EH, Ma SK, Lee TH, Kim SW.
Peroxiredoxin V (PrdxV) negatively regulates EGFR/Stat3-mediated fibrogenesis via a Cys48-dependent interaction between PrdxV and Stat3.
Sci Rep. 2019 Jun19;9(1):8751.
doi: 10.1038/s41598-019-45347-0.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Tamm Horsfall Glycoprotein Antibody gallery image 3
Published customer image:
Sheep anti Human Tamm Horsfall glycoprotein antibody (8595-0054) used to evaluate renal Tamm Horsfall (uromodulin) expression following unilateral ureteral obstruction by western blotting.
Image caption:
Kidney UMOD protein levels increase in UUO‐induced injury. Total kidney UMOD protein levels increased significantly 7, 14, and 21 days after UUO (n = 7/group) compared with the sham group (n = 5/group) in the UMOD+/+ mice, as measured by western blotting (A). UMOD protein was not detected in any of the UMOD‐/‐ mice.

From:Maydan O, McDade PG, Liu Y, Wu XR, Matsell DG, Eddy AA.
Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy.
Physiol Rep. 2018 Mar;6(6):e13654.
doi: 10.14814/phy2.13654.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Tamm Horsfall Glycoprotein Antibody gallery image 4
Published customer image:
Sheep anti Human Tamm Horsfall glycoprotein antibody (8595-0054) used to evaluate renal Tamm horsfall (uromodulin) expression following unilateral ureteral obstruction by immunofluorescence.
Image caption:
Dual staining for UMOD (green) and KIM‐1 (red) in a UMOD+/+ day 14 kidney illustrates an area of tubular intraluminal staining for both proteins (yellow; highlighted by the arrow) (E). Dual staining for UMOD (green) and the proximal tubular apical membrane protein LRP2 (red) in a UMOD+/+ day 14 kidney illustrates the presence of luminal UMOD deposits surrounded by LRP2 + tubules (arrows) (F). By dual staining fluorescence microscopy, TRPV5 (red) was almost exclusively found in tubules with intraluminal UMOD deposits (green), as shown for a day 14 UUO UMOD+/+ kidney (D). A dual stained immunofluorescence photomicrograph illustrates intraluminal areas that express both UMOD (green) and M30 (red) (arrows; C). Photomicrograph magnification: ×40. Scale bars: 25 μm.

From:Maydan O, McDade PG, Liu Y, Wu XR, Matsell DG, Eddy AA.
Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy.
Physiol Rep. 2018 Mar;6(6):e13654.
doi: 10.14814/phy2.13654.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Tamm Horsfall Glycoprotein Antibody gallery image 5
Published customer image:
Sheep anti Human Tamm horsfall glycoprotein antibody (8595-0054) used to evaluate renal Tamm horsfall (uromodulin) expression following unilateral ureteral obstruction by western blotting.
Image caption:
UMOD reduces tubular cell KIM‐1 levels in vitro. Preliminary studies to maximize the exposure of KIM‐1 producing 769‐P cells to UMOD demonstrate that both 769‐P and 293 cells transiently transfected with a pcDNA3.1 vector expressing human UMOD (pcDNA3.1‐UMOD) produced readily detectable UMOD protein levels within cells and their conditioned medium compared with cells transfected with a pcDNA3.1 vector expressing a noncoding control vector (pcDNA3.1‐C).

From:Maydan O, McDade PG, Liu Y, Wu XR, Matsell DG, Eddy AA.
Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy.
Physiol Rep. 2018 Mar;6(6):e13654.
doi: 10.14814/phy2.13654.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Tamm Horsfall Glycoprotein Antibody gallery image 6
Published customer image:
Sheep anti Tamm Horsfall glycoprotein antibody (8595-0054) used to demonstrate uromodulin expression in murine kidney sections by immunohistochemistry.
Image caption:
Micrographs of UMOD expression in TAL of GlatmTg(CAG-A4GALT) and WT mice. Arrows indicate a vacuolated TAL cell. Scale bars, 10 μm.

From: Maruyama, H. et al.
Medullary thick ascending limb impairment in the GlatmTg(CAG-A4GALT) Fabry model mice
FASEB J. 2018 Aug; 32(8): 4544–59.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Tamm Horsfall Glycoprotein Antibody gallery image 7
Published customer image:
Sheep anti Tamm Horsfall glycoprotein antibody (8595-0054) used to demonstrate uromodulin expression in murine kidney sections by immunohistochemistry.
Image caption:
GlatmTg(CAG-A4GALT) mice demonstrate reduced expression of ion transport–related molecules in the TAL. Representative images of UMOD immunoreactivity in kidneys of GlatmTg(CAG-A4GALT) and WT mice (n = 3/group). Scale bars, 1 mm.

From: Maruyama, H. et al.
Medullary thick ascending limb impairment in the GlatmTg(CAG-A4GALT) Fabry model mice
FASEB J. 2018 Aug; 32(8): 4544–59.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Tamm Horsfall Glycoprotein Antibody gallery image 8
Published customer image:
Sheep anti Tamm Horsfall glycoprotein antibody (8595-0054) used to evaluate uromodulin expression in murine kidney by western blotting.
Image caption:
GlatmTg(CAG-A4GALT) mice demonstrate reduced expression of ion transport–related molecules in the TAL. Representative images of UMOD immunoreactivity in kidneys of GlatmTg(CAG-A4GALT) and WT mice (n = 3/group). Scale bars, 1 mm.

From: Maruyama, H. et al.
Medullary thick ascending limb impairment in the GlatmTg(CAG-A4GALT) Fabry model mice
FASEB J. 2018 Aug; 32(8): 4544–59.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Tamm Horsfall Glycoprotein Antibody gallery image 9
Published customer image:
Sheep anti Tamm Horsfall glycoprotein antibody (8595-0054) used to demonstrate uromodulin expression in murine kidney sections by immunohistochemistry.
Image caption:
Micrographs of kidney biopsy serial sections (from patients with Fabry nephropathy. Anti-UMOD Ab, Impaired cells displayed vacuolization by PAS staining and attenuated or negative immunoreactivity (arrowheads). Fabry nephropathy patients were naive to enzyme replacement therapy at the time of kidney biopsy. Scale bars, 20 μm.

From: Maruyama, H. et al.
Medullary thick ascending limb impairment in the GlatmTg(CAG-A4GALT) Fabry model mice
FASEB J. 2018 Aug; 32(8): 4544–59.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Sheep anti Human Tamm Horsfall Glycoprotein

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
8595-0054
Datasheet
C E IF
SDS
1 ml loader

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
IgG fraction - liquid
Preparation
IgG fraction prepared by anion exchange chromatography
Buffer Solution
Glycine buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
0.1%EACA
0.01%Benzamidine
1 mMEDTA

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P07911
Entrez Gene
UMOD
GO Terms
GO:0005509 calcium ion binding
GO:0005576 extracellular region
GO:0006968 cellular defense response
GO:0008285 negative regulation of cell proliferation
GO:0031225 anchored to membrane
GO:0016324 apical plasma membrane
GO:0019898 extrinsic to membrane
Regulatory
For research purposes only

Applications of Tamm Horsfall Glycoprotein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/40,000
Immunofluorescence
Immunohistology - Frozen 1/50 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
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Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for Tamm Horsfall Glycoprotein antibody

  1. El-Achkar, T.M. et al. (2011) Tamm-Horsfall protein-deficient thick ascending limbs promote injury to neighboring S3 segments in an MIP-2-dependent mechanism.
    Am J Physiol Renal Physiol. 300 (4): F999-1007.
  2. Hao, S. et al. (2011) Differential regulation of NFAT5 by NKCC2 isoforms in medullary thick ascending limb (mTAL) cells.
    Am J Physiol Renal Physiol. 300: F966-75.
  3. Vekaria, R.M. et al. (2006) Immunolocalization of ectonucleotidases along the rat nephron.
    Am J Physiol Renal Physiol. 290 (2): F550-60.
  4. El-Achkar, T.M. et al. (2013) Tamm-Horsfall protein translocates to the basolateral domain of thick ascending limbs, interstitium, and circulation during recovery from acute kidney injury.
    Am J Physiol Renal Physiol. 304 (8): F1066-75.
  5. El-Achkar, T.M. et al. (2008) Tamm-Horsfall protein protects the kidney from ischemic injury by decreasing inflammation and altering TLR4 expression.
    Am J Physiol Renal Physiol. 295 (2): F534-44.
  6. Nevo, N. et al. (2010) Renal phenotype of the cystinosis mouse model is dependent upon genetic background.
    Nephrol Dial Transplant. 25: 1059-66.
  7. Venables, G. et al. (2011) Ghrelin receptors are expressed by distal tubules of the mouse kidney.
    Cell Tissue Res. 346: 135-9.
  8. Rice, J.C. et al. (2002) Monocyte chemoattractant protein-1 expression correlates with monocyte infiltration in the post-ischemic kidney.
    Ren Fail. 24: 703-23.
  9. Liu, S. et al. (2012) Autophagy plays a critical role in kidney tubule maintenance, aging and ischemia-reperfusion injury.
    Autophagy. 8: 826-37.
  10. Saito, S. et al. (2011) Analysis of glial cell line-derived neurotrophic factor-inducible zinc finger protein 1 expression in human diseased kidney.
    Hum Pathol. 42: 848-58.
  11. Ferrè, S. et al. (2014) Mutations in PCBD1 cause hypomagnesemia and renal magnesium wasting.
    J Am Soc Nephrol. 25 (3): 574-86.
  12. Maeda-Hori, M. et al. (2014) Plasma CD147 reflects histological features in patients with lupus nephritis.
    Lupus. 23: 342-52.
  13. Maruyama, H. et al. (2018) Medullary thick ascending limb impairment in the GlatmTg(CAG-A4GALT) Fabry model mice.
    FASEB J. 32 (8): 4544-59.
  14. Choi, H.I. et al. (2019) Peroxiredoxin V (PrdxV) negatively regulates EGFR/Stat3-mediated fibrogenesis via a Cys48-dependent interaction between PrdxV and Stat3.
    Sci Rep. 9 (1): 8751.
  15. Maydan, O. et al. (2018) Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy.
    Physiol Rep. 6 (6): e13654.
  16. Heitmeier, M. et al. (2014) The role of tumor necrosis factor alpha in regulating the expression of Tamm-Horsfall Protein (uromodulin) in thick ascending limbs during kidney injury.
    Am J Nephrol. 40 (5): 458-67.

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