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Tamm Horsfall Glycoprotein antibody

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Sheep anti Human Tamm Horsfall Glycoprotein

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
8595-0054
Datasheet
C E IF
MSDS
1 ml loader

Sheep anti Human Tamm Horsfall Glycoprotein antibody recognizes human Tamm Horsfall Protein (THP), also known as uromodulin (Pennica et al. 1987). THP was originally isolated from normal human urine (Tamm et al. 1952). THP is a 616 amino acid ~85 kDa glycoprotein synthesized by the kidney and is the most abundant urinary protein in healthy individuals (Pennica et al. 1987). THP forms the matrix of all urinary casts either alone (hyaline casts) or in association with cellular fragments and filtered proteins (granular casts), casts are indicative of a number of pathological states including glomerulonephritis and vasculitis (Chambers et al. 1986).

The specificity of sheep anti human Tamm Horsfall glycoprotein antibody has been confirmed by double diffusion against tamm horsfall glycoprotein and an independent known antibody against THP.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
IgG fraction - liquid
Preparation
IgG fraction prepared by ammonium sulphate fractionation
Buffer Solution
Glycine buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
0.1%EACA
0.01%Benzamidine
1 mMEDTA
Immunogen
Purified human uromucodulin prepared from urine.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P07911 Related reagents
Entrez Gene
UMOD Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0005576 extracellular region
GO:0006968 cellular defense response
GO:0008285 negative regulation of cell proliferation
GO:0031225 anchored to membrane
GO:0016324 apical plasma membrane
GO:0019898 extrinsic to membrane
Regulatory
For research purposes only

Applications of Tamm Horsfall Glycoprotein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/40,000
Immunofluorescence
Immunohistology - Frozen 1/50 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:FITC STAR88F C 1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Application Based External Images

Immunofluorescence

Product Specific References

References for Tamm Horsfall Glycoprotein antibody

  1. El-Achkar, T.M. et al. (2011) Tamm-Horsfall protein-deficient thick ascending limbs promote injury to neighboring S3 segments in an MIP-2-dependent mechanism.
    Am J Physiol Renal Physiol. 300 (4): F999-1007.
  2. Hao, S. et al. (2011) Differential regulation of NFAT5 by NKCC2 isoforms in medullary thick ascending limb (mTAL) cells.
    Am J Physiol Renal Physiol. 300: F966-75.
  3. Vekaria, R.M. et al. (2006) Immunolocalization of ectonucleotidases along the rat nephron.
    Am J Physiol Renal Physiol. 290 (2): F550-60.
  4. El-Achkar, T.M. et al. (2013) Tamm-Horsfall protein translocates to the basolateral domain of thick ascending limbs, interstitium, and circulation during recovery from acute kidney injury.
    Am J Physiol Renal Physiol. 304 (8): F1066-75.
  5. El-Achkar, T.M. et al. (2008) Tamm-Horsfall protein protects the kidney from ischemic injury by decreasing inflammation and altering TLR4 expression.
    Am J Physiol Renal Physiol. 295 (2): F534-44.
  6. Nevo, N. et al. (2010) Renal phenotype of the cystinosis mouse model is dependent upon genetic background.
    Nephrol Dial Transplant. 25: 1059-66.
  7. Venables, G. et al. (2011) Ghrelin receptors are expressed by distal tubules of the mouse kidney.
    Cell Tissue Res. 346: 135-9.
  8. Rice, J.C. et al. (2002) Monocyte chemoattractant protein-1 expression correlates with monocyte infiltration in the post-ischemic kidney.
    Ren Fail. 24: 703-23.
  9. Liu, S. et al. (2012) Autophagy plays a critical role in kidney tubule maintenance, aging and ischemia-reperfusion injury.
    Autophagy. 8: 826-37.
  10. Saito, S. et al. (2011) Analysis of glial cell line-derived neurotrophic factor-inducible zinc finger protein 1 expression in human diseased kidney.
    Hum Pathol. 42: 848-58.
  11. Ferrè, S. et al. (2014) Mutations in PCBD1 cause hypomagnesemia and renal magnesium wasting.
    J Am Soc Nephrol. 25 (3): 574-86.
  12. Maeda-Hori, M. et al. (2014) Plasma CD147 reflects histological features in patients with lupus nephritis.
    Lupus. 23: 342-52.
  13. Maruyama, H. et al. (2018) Medullary thick ascending limb impairment in the GlatmTg(CAG-A4GALT) Fabry model mice.
    FASEB J. : fj201701374R.
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