TAGLN antibody

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Goat anti TAGLN

TAGLN antibody recognizes human transgelin, also known as 22 kDa actin-binding protein, protein WS3-10, smooth muscle protein 22-alpha or SM22-alpha. The antibody has been extensively validated for western blotting using whole cell lysates.

TAGLN Antibody with Control Lysate

TAGLN antibody recognizes human transgelin, also known as 22 kDa actin-binding protein, protein WS3-10, smooth muscle protein 22-alpha or SM22-alpha. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00048 WB 100 µl
VPA00048KT WB 2 Westerns
With Lysate 400µg
Goat anti Human TAGLN antibody recognizes human TAGLN (transgelin), otherwise known as SM22-alpha. TAGLN is a shape change sensitive, actin cross-linking/gelling protein, and a member of the calponin family. TAGLN plays a role in calcium interactions and contractile properties of the cell.

TAGLN is ubiquitously expressed by vascular and visceral smooth muscle, is an early marker of smooth muscle differentiation and is overexpressed by senescent human fibroblasts. Studies have identified TAGLN as a novel tumor suppressor protein, which has a markedly reduced expression in colorectal cancer samples, and may serve as an important biomarker of malignancy.

Goat anti Human TAGLN antibody recognizes TAGLN as a band of 22 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-MTGYGRPRQIIS from the C-terminal region of TAGLN.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q01995 Related reagents
Entrez Gene
TAGLN Related reagents
GO Terms
GO:0003779 actin binding
GO:0005737 cytoplasm
GO:0007517 muscle organ development
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of TAGLN antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Goat anti TAGLN detects a band of approximately 22 kDa in HeLa cell lysates.
Western Blotting
Goat anti TAGLN detects a band of approximately 22 kDa in HeLa cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB