TAGLN antibody

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Goat anti Human TAGLN (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2034 E WB 0.1 mg
Goat anti Human TAGLN antibody recognizes human Transgelin, also known as TAGLN, a ~22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha or SM22-alpha, Transgelin is a shape change sensitive, actin cross-linking/gelling protein, and member of the calponin family, which plays a role in calcium interactions and contractile properties of the cell.

TAGLN is ubiquitously expressed by vascular and visceral smooth muscle, and an early marker of smooth muscle differentiation, and also overexpressed by senescent human fibroblasts. Transgelin has been identified as a novel tumor suppressor protein, which has a markedly reduced expression in colorectal cancer samples, and may serve as an important biomarker of malignancy (Zhang et al. 2010).

Goat anti Human TAGLN antibody recognizes an epitope within the C-terminal (CT) region of human transgelin.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
Bovine Expected from Sequence
Dog Expected from Sequence
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human TAGLN (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-MTGYGRPRQIIS from the C-terminal region of TAGLN (NP_003177.2; NP_001001522.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q01995 Related reagents
Entrez Gene
TAGLN Related reagents
GO Terms
GO:0003779 actin binding
GO:0005737 cytoplasm
GO:0007517 muscle organ development
Regulatory
For research purposes only

Applications of TAGLN antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1:2000
Western Blotting 0.001 0.003ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2034 detects a band of approximately 23kDa in human placenta, heart and testis cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

References for TAGLN antibody

  1. Lea, R.G. et al. (2016) The fetal ovary exhibits temporal sensitivity to a 'real-life' mixture of environmental chemicals.
    Sci Rep. 6: 22279.
  2. Huuskonen, P. et al. (2016) The human placental proteome is affected by maternal smoking.
    Reprod Toxicol. 63: 22-31.