SURF1 antibody

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Anti SURF1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with SURF1 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 80 second exposure. Arrow points to SURF1 (molecular weight 36 kDa)

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Rabbit anti SURF1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: SURF1

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VPA00374 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human SURF1 antibody recognizes the SURF1, also known as surfeit locus protein 1 or surfeit 1.

The SURF1 gene encodes a protein localized to the inner mitochondrial membrane and thought to be involved in the biogenesis of the cytochrome c oxidase complex. The protein is a member of the SURF1 family, which includes the related yeast protein SHY1 and rickettsial protein RP733. The gene is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity, where it shares a bidirectional promoter with SURF2 on the opposite strand. Defects in SURF1 are a cause of Leigh syndrome, a severe neurological disorder that is commonly associated with systemic cytochrome c oxidase deficiency (provided by RefSeq, Jul 2008).

Rabbit anti Human SURF1 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti SURF1 detects a band of approximately 36 kDa in HepG2 cell lysate
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose
Immunogen: Synthetic peptide corresponding to the N-terminal region of human SURF1
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20^oC, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q15526
Entrez Gene: SURF1
GO Terms: GO:0004129 cytochrome-c oxidase activity; GO:0016021 integral to membrane; GO:0005746 mitochondrial respiratory chain; GO:0008535 respiratory chain complex IV assembly; GO:0009060 aerobic respiration