Superoxide Dismutase (MN) antibody
Goat anti Superoxide Dismutase (MN)
Superoxide dismutase [Mn] recognizes manganese superoxide dismutase (MNSOD) or SOD2. The antibody has been extensively validated for western blotting using whole cell lysates.
Superoxide Dismutase (MN) Antibody with Control Lysate
Superoxide dismutase [Mn] recognizes manganese superoxide dismutase (MNSOD) or SOD2. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00069 | WB | 100 µl | ||||
| VPA00069KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human superoxide dismutase (Mn) antibody recognizes manganese superoxide dismutase (MNSOD) or SOD2, a superoxide dismutase with manganese at the active site. Superoxide dismutases (SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. There are three major families of superoxide dismutase, depending on the metal cofactor: Cu-Zn (which binds both copper and zinc), Fe and Mn types (which bind either iron or manganese), and finally the Ni type, which binds nickel.
Goat anti Human superoxide dismutase (Mn) antibody recognises human superoxide dismutase (Mn) as a single band of 24 kDa in range of human cell line lysates.
Goat anti Human superoxide dismutase (Mn) antibody recognises human superoxide dismutase (Mn) as a single band of 24 kDa in range of human cell line lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Goat anti superoxide dismutase (Mn) detects a band of approximately 24 kDa in K562 cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide with sequence C-EAIKRDFGSFDK from the internal region of the protein.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P04179 Related reagents
- Entrez Gene
- SOD2 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0001315 age-dependent response to reactive oxygen species
- GO:0001836 release of cytochrome c from mitochondria
- GO:0003069 vasodilation by acetylcholine involved in regulation of systemic arterial blood pressure
- GO:0004784 superoxide dismutase activity
- GO:0005759 mitochondrial matrix
- GO:0006357 regulation of transcription from RNA polymerase II promoter
- GO:0019430 removal of superoxide radicals
- GO:0030145 manganese ion binding
- GO:0032364 oxygen homeostasis
- GO:0043524 negative regulation of neuron apoptosis
- GO:0051289 protein homotetramerization
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Superoxide Dismutase (MN) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
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