Substance P antibody

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Rabbit anti Human Substance P

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
8450-0004 C IF P* 0.5 ml
Rabbit anti Human Substance P antibody recognizes human Protachykinin-1, also known as PPT. Protachykinin-1 is a 129 amino acid ~15kDa tachychinin involved in the excitation of neurons. Protachykinin-1 can be cleaved into a number of chains including substance P, neurokinin A, neuropeptide K, neuropeptide γ and c-terminal flankning peptide. Rabbit anti Human Substance P antibody recognizes an epitope withing the substance P region of the molecule. There are potentially four isoforms of Protachykinin-1 (α - δ) formed by alternative splicing, all containing the substance P peptide region (UniProt: P20366).

Rabbit anti Human Substance P antibody stains substance P secreting cells of the external median eminance of the hypothalamus and specific cells of the GI tract. Rabbit anti Human Substance P antibody also stains neuronal structures.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Synthetic substance P conjugated to Keyhole limpet hemocyanin.
Approx. Protein Concentrations
5.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P20366 Related reagents
Entrez Gene
TAC1 Related reagents
Regulatory
For research purposes only

Applications of Substance P antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen 1/50 1/200
Immunohistology - Paraffin 1 1/50 1/200
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Appendix

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

References for Substance P antibody

  1. Cheng, C. et al. (2010) Dynamic Plasticity of Axons within a Cutaneous Milieu.
    J. Neurosci. 30: 14735-44.
  2. Nguyen, J. et al. (2014) Morphine stimulates cancer progression and mast cell activation and impairs survival in transgenic mice with breast cancer.
    Br J Anaesth. pii: aeu090.
  3. Jouml;nsson, M. et al. (2005) Substance P and the neurokinin-1 receptor in relation to eosinophilia in ulcerative colitis.
    Peptides. 26: 799-814.
  4. Bjur, D. et al. (2005) The innervation pattern of the human Achilles tendon: studies of the normal and tendinosis tendon with markers for general and sensory innervation.
    Cell Tissue Res. 320: 201-6.
  5. Andersson, G. et al. (2007) Nerve-related characteristics of ventral paratendinous tissue in chronic Achilles tendinosis.
    Knee Surg Sports Traumatol Arthrosc. 15: 1272-9.
  6. Danielson, P. et al. (2006) Distribution of general (PGP 9.5) and sensory (substance P/CGRP) innervations in the human patellar tendon.
    Knee Surg Sports Traumatol Arthrosc. 14: 125-32.
  7. Grimsholm, O. et al. (2008) Observations favouring the occurrence of local production and marked effects of bombesin/gastrin-releasing peptide in the synovial tissue of the human knee joint – Comparisons with substance P and the NK-1 receptor.
    Neuropeptides. 42: 133-45.
  8. Grimsholm, O. et al. (2007) Are neuropeptides important in arthritis? Studies on the importance of bombesin/GRP and substance P in a murine arthritis model.
    Ann N Y Acad Sci. 1110: 525-38.
  9. Danielson, P. et al. (2008) Marked sympathetic component in the perivascular innervation of the dorsal paratendinous tissue of the patellar tendon in arthroscopically treated tendinosis patients.
    Knee Surg Sports Traumatol Arthrosc. 16: 621-6.
  10. Backman, L.J. et al. (2011) Substance P is a mechanoresponsive, autocrine regulator of human tenocyte proliferation.
    PLoS One. 6: e27209.
  11. Danielson, P. et al. (2007) Studies on the importance of sympathetic innervation, adrenergic receptors, and a possible local catecholamine production in the development of patellar tendinopathy (tendinosis) in man.
    Microsc Res Tech. 70: 310-24.
  12. Mousavizadeh, R. et al. (2014) Dexamethasone decreases substance P expression in human tendon cells: an in vitro study.
    Rheumatology (Oxford). pii: keu315.
  13. Pinto, F.M. et al. (2015) Analysis of the Expression of Tachykinins and Tachykinin Receptors in the Rat Uterus During Early Pregnancy.
    Biol Reprod. pii: biolreprod.115.130617.
  14. González-Santana, A. et al. (2016) Altered expression of the tachykinins substance P/neurokinin A/hemokinin-1 and their preferred neurokinin 1/neurokinin 2 receptors in uterine leiomyomata.
    Fertil Steril. 106 (6): 1521-9.