Stem Cell Factor antibody

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Goat anti Human Stem Cell Factor

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1210 E FN P* WB 0.1 mg
Goat anti Human Stem Cell Factor antibody recognizes human stem cell factor (SCF), a type I transmembrane protein and haematopoietic growth factor, which acts in synergy with other growth factors and signals through the c-Kit receptor.

Interaction between SCF and the receptor tyrosine kinase c-Kit is an essential step for the proliferation, survival and differentiation of haematopoietic progenitor cells, mast cells, myelanocytes and germ cells, and plays an important role in follicular development. The association between SCF and c-Kit is also involved with the progression and invasiveness of tumours, and deficiencies of either SCF or c-Kit result in mast cell deficiency and macrocytic anaemia.

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human stem cell factor were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human stem cell factor.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt
P21583 Related reagents
Entrez Gene
KITLG Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0007165 signal transduction
GO:0005173 stem cell factor receptor binding
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0008083 growth factor activity
GO:0008283 cell proliferation
GO:0008584 male gonad development
GO:0042802 identical protein binding
GO:0030097 hemopoiesis
GO:0045740 positive regulation of DNA replication
Regulatory
For research purposes only

Applications of Stem Cell Factor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 0.008 0.012ug/ml
Immunohistology - Paraffin 1
Western Blotting 0.1 0.2ug/ml
  1. 1 This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
    Successful heat mediated antigen retrieval has also been accomplished using Tris/EDTA buffer (pH9.0).
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
AHP1210 may be used in an indirect ELISA with PHP067 as the standard.
Western Blotting
AHP1210 may be used for Western blotting with PHP067 as the positive control.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

Further Reading

  1. O'laughlin-bunner, B. et al. (2001) Lyn is required for normal stem cell factor-induced proliferation and chemotaxis of primary hematopoietic cells.
    Blood. 98 (2): 343-50.
  2. Rönnstrand, L. (2004) Signal transduction via the stem cell factor receptor/c-Kit.
    Cell Mol Life Sci. 61 (19-20): 2535-48.