Stem Cell Factor antibody

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Anti Human Stem Cell Factor Antibody thumbnail image 2

Anti Human Stem Cell Factor Antibody thumbnail image 3

Anti Human Stem Cell Factor Antibody thumbnail image 4

Anti Human Stem Cell Factor Antibody gallery image 1 — Formalin fixed, paraffin embedded human pancreatic adenocarcinoma stained with Rabbit anti Human SCF antibody (**AHP1210**) following enzyme mediated antigen retrieval using proteinase K

Anti Human Stem Cell Factor Antibody gallery image 2 — Formalin fixed, paraffin embedded human pancreatic adenocarcinoma stained with Rabbit anti Human SCF antibody (**AHP1210**) following enzyme mediated antigen retrieval using proteinase K.

Anti Human Stem Cell Factor Antibody gallery image 3 — Formalin fixed, paraffin embedded human pancreatic adenocarcinoma stained with Rabbit anti Human SCF antibody (**AHP1210**) following heat mediated antigen retrieval using EDTA buffer (pH9.0)

Anti Human Stem Cell Factor Antibody gallery image 4 — Formalin fixed, paraffin embedded human pancreatic adenocarcinoma stained with Rabbit anti Human SCF antibody (**AHP1210**) following enzyme mediated antigen retrieval using proteinase K

Goat anti Human Stem Cell Factor

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1210	E FN P* WB			0.1 mg

Goat anti Human Stem Cell Factor antibody recognizes human stem cell factor (SCF), a type I transmembrane protein and haematopoietic growth factor, which acts in synergy with other growth factors and signals through the c-Kit receptor.

Interaction between SCF and the receptor tyrosine kinase c-Kit is an essential step for the proliferation, survival and differentiation of haematopoietic progenitor cells, mast cells, myelanocytes and germ cells, and plays an important role in follicular development. The association between SCF and c-Kit is also involved with the progression and invasiveness of tumours, and deficiencies of either SCF or c-Kit result in mast cell deficiency and macrocytic anaemia.

Product Details

Target Species: Human
Product Form: Purified IgG - lyophilised
Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation: Antisera to human stem cell factor were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Immunogen: Recombinant human stem cell factor.
Approx. Protein Concentrations: IgG concentration 1.0mg/ml

Storage Information

Storage: Prior to reconstitution store at -20^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt: P21583 Related reagents
Entrez Gene: KITLG Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0007155 cell adhesion; GO:0016021 integral to membrane; GO:0007165 signal transduction; GO:0005173 stem cell factor receptor binding; GO:0005737 cytoplasm; GO:0005856 cytoskeleton; GO:0008083 growth factor activity; GO:0008283 cell proliferation; GO:0008584 male gonad development; GO:0042802 identical protein binding; GO:0030097 hemopoiesis; GO:0045740 positive regulation of DNA replication
Regulatory: For research purposes only

Applications of Stem Cell Factor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.5	2.0ug/ml
Functional Assays	0.008	0.012ug/ml
Immunohistology - Paraffin ¹
Western Blotting	0.1	0.2ug/ml

¹ This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
Successful heat mediated antigen retrieval has also been accomplished using Tris/EDTA buffer (pH9.0).

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: AHP1210 may be used in an indirect ELISA with PHP067 as the standard.
Western Blotting: AHP1210 may be used for Western blotting with PHP067 as the positive control.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	1 mg	F
Rabbit anti Goat IgG (Fc):HRP	STAR122P	1 mg	C E WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P