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STEAP1 antibody

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Anti STEAP1 (C-Terminal) Antibody thumbnail image 1 Anti STEAP1 (C-Terminal) Antibody thumbnail image 2
Anti STEAP1 (C-Terminal) Antibody gallery image 1
Western blot analysis of human spleen lysate probed with Rabbit ati Human STEAP1 (AHP1438) at 1(A) and 2(B) ug/ml
Anti STEAP1 (C-Terminal) Antibody gallery image 2
Formalin fixed, paraffin embedded human spleen stained with Rabbit anti Human STEAP1 (AHP1438) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

Rabbit anti STEAP1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1438 P* WB 0.1 mg
Rabbit anti Human STEAP1 antibody recognizes STEAP1, also known as six transmembrane epithelial antigen of the prostate. STEAP1 is a ~39 kDa protein and member of a family of cell surface serpentine transmembrane antigens of the prostate.

STEAP1 is predominantly expressed in human prostate and is up-regulated in several cancer lines including prostate, ovarian, cervical, breast, testicular, bladder, colon and Ewing sarcoma. It is significantly expressed at cell-cell junctions and has been shown to be a target of human CD8 T cells. This indicates that it could be used as a potential target for cancer immunotherapy. This antibody does not cross-react with other STEAP-proteins.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antisera to human STEAP (CT) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
An 18 amino acid peptide located near the c-terminal region of human STEAP1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9UHE8 Related reagents
Entrez Gene
STEAP1 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0005887 integral to plasma membrane
GO:0005506 iron ion binding
GO:0005911 cell-cell junction
GO:0016491 oxidoreductase activity
GO:0022900 electron transport chain
GO:0006826 iron ion transport
GO:0010008 endosome membrane
GO:0015267 channel activity
GO:0050660 flavin adenine dinucleotide binding
Regulatory
For research purposes only

Applications of STEAP1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 1 2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1438 detects a band of approximately 40kDa in human spleen cell lysates.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Hubert, R.S. et al. (1999) STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumors.
    Proc Natl Acad Sci U S A. 96 (25): 14523-8.
  2. Alves, P.M. et al. (2006) STEAP, a prostate tumor antigen, is a target of human CD8+ T cells.
    Cancer Immunol Immunother. 55 (12): 1515-23.
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