STEAP1 antibody


STEAP1 is predominantly expressed in human prostate and is up-regulated in several cancer lines including prostate, ovarian, cervical, breast, testicular, bladder, colon and Ewing sarcoma. It is significantly expressed at cell-cell junctions and has been shown to be a target of human CD8 T cells. This indicates that it could be used as a potential target for cancer immunotherapy. This antibody does not cross-react with other STEAP-proteins.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Antisera to human STEAP (CT) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- An 18 amino acid peptide located near the c-terminal region of human STEAP1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- Q9UHE8 Related reagents
- Entrez Gene
- STEAP1 Related reagents
- GO Terms
- GO:0009055 electron carrier activity
- GO:0005887 integral to plasma membrane
- GO:0005506 iron ion binding
- GO:0005911 cell-cell junction
- GO:0016491 oxidoreductase activity
- GO:0022900 electron transport chain
- GO:0006826 iron ion transport
- GO:0010008 endosome membrane
- GO:0015267 channel activity
- GO:0050660 flavin adenine dinucleotide binding
- Regulatory
- For research purposes only
Applications of STEAP1 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Paraffin 1 | |||
Western Blotting | 1 | 2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Western Blotting
- AHP1438 detects a band of approximately 40kDa in human spleen cell lysates.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Sheep anti Rabbit IgG:Biotin | 2AB02B | C E P WB | 1 ml |
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Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Hubert, R.S. et al. (1999) STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumors.
Proc Natl Acad Sci U S A. 96 (25): 14523-8. -
Alves, P.M. et al. (2006) STEAP, a prostate tumor antigen, is a target of human CD8+ T cells.
Cancer Immunol Immunother. 55 (12): 1515-23.