STEAP1 antibody

Rabbit anti STEAP1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
STEAP1
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1438
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Human STEAP1 antibody recognizes STEAP1, also known as six transmembrane epithelial antigen of the prostate. STEAP1 is a ~39 kDa protein and member of a family of cell surface serpentine transmembrane antigens of the prostate.

STEAP1 is predominantly expressed in human prostate and is up-regulated in several cancer lines including prostate, ovarian, cervical, breast, testicular, bladder, colon and Ewing sarcoma. It is significantly expressed at cell-cell junctions and has been shown to be a target of human CD8 T cells. This indicates that it could be used as a potential target for cancer immunotherapy. This antibody does not cross-react with other STEAP-proteins.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antisera to human STEAP (CT) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
An 18 amino acid peptide located near the c-terminal region of human STEAP1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 1 2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1438 detects a band of approximately 40kDa in human spleen cell lysates.

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Further Reading

  1. Hubert, R.S. et al. (1999) STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumors.
    Proc Natl Acad Sci U S A. 96 (25): 14523-8.
  2. Alves, P.M. et al. (2006) STEAP, a prostate tumor antigen, is a target of human CD8+ T cells.
    Cancer Immunol Immunother. 55 (12): 1515-23.

Synonyms
STEAP
RRID
AB_2271260
UniProt
Q9UHE8
Entrez Gene
STEAP1
GO Terms
GO:0009055 electron carrier activity
GO:0005887 integral to plasma membrane
GO:0005506 iron ion binding
GO:0005911 cell-cell junction
GO:0016491 oxidoreductase activity
GO:0022900 electron transport chain
GO:0006826 iron ion transport
GO:0010008 endosome membrane
GO:0015267 channel activity
GO:0050660 flavin adenine dinucleotide binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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