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STAT5A (pTyr694) antibody

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Anti STAT5A (pTyr694) Antibody thumbnail image 1 Anti STAT5A (pTyr694) Antibody thumbnail image 2
Anti STAT5A (pTyr694) Antibody gallery image 1
Western blot analysis of extracts from K562 cells untreated or treated with G-CSF using Rabbit anti STAT5A (pTyr694) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with G-CSF (25ng/ml) for 30 minutes
Anti STAT5A (pTyr694) Antibody gallery image 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti STAT5A (pTyr694) antibody

Rabbit anti STAT5A (pTyr694)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2689
Datasheet
P WB
SDS
50 µl loader

Rabbit anti STAT5A (pTyr694) antibody recognizes STAT5A, also known as signal transducer and activator of transcription 5A when phosphorylated at tyrosine 694. STAT5A is a member of the STAT family of transcription factors. Members of the STAT family reside in the cytosol until activated. Upon activation, STATs dimerize and translocate to the nucleus where they exert their transcriptional effects. STAT5a is phosphorylated at tyrosine 694 by members of JAK family of kinases as well as receptor tyrosine kinases (Coffer et al. 2000). Phosphorylation at tyrosine 694 is crucial for activation of STAT5a.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to STAT5A (pTyr694) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 694 of human STAT5A

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P42229
Entrez Gene
STAT5A
GO Terms
GO:0005515 protein binding
GO:0000255 allantoin metabolic process
GO:0005509 calcium ion binding
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0006101 citrate metabolic process
GO:0006103 2-oxoglutarate metabolic process
GO:0006105 succinate metabolic process
GO:0006107 oxaloacetate metabolic process
GO:0006549 isoleucine metabolic process
GO:0006573 valine metabolic process
GO:0006600 creatine metabolic process
GO:0006631 fatty acid metabolic process
GO:0019530 taurine metabolic process
GO:0046449 creatinine metabolic process
Regulatory
For research purposes only

Applications of STAT5A (pTyr694) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
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Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Coffer, P.J. et al. (2000) The role of STATs in myeloid differentiation and leukemia.
    Oncogene. 19 (21): 2511-22.
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