STAT5A (pTyr694) antibody
Rabbit anti STAT5A (pTyr694) antibody recognizes STAT5A, also known as signal transducer and activator of transcription 5A when phosphorylated at tyrosine 694. STAT5A is a member of the STAT family of transcription factors. Members of the STAT family reside in the cytosol until activated. Upon activation, STATs dimerize and translocate to the nucleus where they exert their transcriptional effects. STAT5a is phosphorylated at tyrosine 694 by members of JAK family of kinases as well as receptor tyrosine kinases (Coffer et al. 2000). Phosphorylation at tyrosine 694 is crucial for activation of STAT5a.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to STAT5A (pTyr694) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding tyrosine 694 of human STAT5A
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P42229
- Entrez Gene
- STAT5A
- GO Terms
- GO:0005515 protein binding
- GO:0000255 allantoin metabolic process
- GO:0005509 calcium ion binding
- GO:0003677 DNA binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- GO:0006101 citrate metabolic process
- GO:0006103 2-oxoglutarate metabolic process
- GO:0006105 succinate metabolic process
- GO:0006107 oxaloacetate metabolic process
- GO:0006549 isoleucine metabolic process
- GO:0006573 valine metabolic process
- GO:0006600 creatine metabolic process
- GO:0006631 fatty acid metabolic process
- GO:0019530 taurine metabolic process
- GO:0046449 creatinine metabolic process
- Regulatory
- For research purposes only
Applications of STAT5A (pTyr694) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 1/50 | 1/100 | |
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
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| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Coffer, P.J. et al. (2000) The role of STATs in myeloid differentiation and leukemia.
Oncogene. 19 (21): 2511-22.
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