STAT3 (pTyr705) antibody

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Rabbit anti STAT3 (pTyr705)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2686 WB 50 µl
Rabbit anti STAT3 (pTyr705) antibody recognizes STAT3, also known as acute-phase response factor when phosphorylated at tyrosine 705. STAT3 is a member of the STAT family of transcription factors. STAT3 is phosphorylated at tyrosine 705 by JAKs causing homodimerization and translocation to the nucleus (Decker and Kovarik, 2000).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to STAT3 (pTyr705) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 705 of human STAT3

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P40763 Related reagents
Entrez Gene
STAT3 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0005509 calcium ion binding
GO:0001659 temperature homeostasis
GO:0001754 eye photoreceptor cell differentiation
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006606 protein import into nucleus
GO:0005829 cytosol
GO:0004871 signal transducer activity
GO:0005634 nucleus
GO:0006928 cellular component movement
GO:0008134 transcription factor binding
GO:0016563 transcription activator activity
GO:0019901 protein kinase binding
GO:0019953 sexual reproduction
GO:0032355 response to estradiol stimulus
GO:0042593 glucose homeostasis
GO:0042755 eating behavior
GO:0044419 interspecies interaction between organisms
GO:0046983 protein dimerization activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0060397 JAK-STAT cascade involved in growth hormone signaling pathway
GO:0070102 interleukin-6-mediated signaling pathway
Regulatory
For research purposes only

Applications of STAT3 (pTyr705) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Decker, T. & Kovarik, P. (2000) Serine phosphorylation of STATs.
    Oncogene. 19 (21): 2628-37.