STAT3 (pSer727) antibody

100% Secure


Rabbit anti STAT3 (pSer727)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2685
Datasheet
WB
MSDS
50 µl loader

Rabbit anti STAT3 (pSer727) antibody recognizes signal transducer and activator of transcription 3 (STAT3), also known as acute-phase response factor, when phosphorylated at serine 727. STAT3 is a member of the STAT family of transcription factors, playing a key role in numerous processes including cell differentiation, proliferation and transformation. Phosphorylation of STAT3 at tyrosine 705 leads to homo- or heterodimerization of STAT3 and is a critical phosphorylation site for STAT3 activity (Huang et al. 2014).

Serine 727 is also a key phosphorylation site of STAT3. Serine 727 is phosphorylated by multiple kinases including mitogen-activated kinases (MAPK), mammalian target of rapamycin kinase (mTOR), cyclin-dependent kinase 5 (CDK5) and nemo-like kinase (NLK) (Wakahara et al. 2012). Controversy exists around the effect of phosphorylation at serine 727 (Wakahara et al. 2012). There are reports that the transcriptional activity of STAT3 coincides with phosphorylation at serine 727. Some reports indicate that the transcriptional activity of STAT3 coincides with phosphorylation at serine 727 (Yokogami et al. 2000). Other reports show STAT3 activation independent of serine 727 (Wakahara et al. 2012). The role of serine 727 is likely dependent on the larger cellular context.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to STAT3 (pSer727) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 727 of human STAT3

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P40763
Entrez Gene
STAT3
GO Terms
GO:0005886 plasma membrane
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0005509 calcium ion binding
GO:0001659 temperature homeostasis
GO:0001754 eye photoreceptor cell differentiation
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006606 protein import into nucleus
GO:0005829 cytosol
GO:0004871 signal transducer activity
GO:0005634 nucleus
GO:0006928 cellular component movement
GO:0008134 transcription factor binding
GO:0016563 transcription activator activity
GO:0019901 protein kinase binding
GO:0019953 sexual reproduction
GO:0032355 response to estradiol stimulus
GO:0042593 glucose homeostasis
GO:0042755 eating behavior
GO:0044419 interspecies interaction between organisms
GO:0046983 protein dimerization activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0060397 JAK-STAT cascade involved in growth hormone signaling pathway
GO:0070102 interleukin-6-mediated signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of STAT3 (pSer727) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Huang, G. et al. (2014) STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates mouse ESC fates.
    Stem Cells. 32 (5): 1149-60.
  2. Wakahara, R. et al. (2012) Phospho-Ser727 of STAT3 regulates STAT3 activity by enhancing dephosphorylation of phospho-Tyr705 largely through TC45.
    Genes Cells. 17 (2): 132-45.