STAT1 Alpha antibody

ISO 9001 Quality Assurance Manufacturing | Print

Anti STAT1 Alpha Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Rabbit anti Human STAT1 alpha antibody (**VPA00008**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/50,000, STAR208P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to STAT1 alpha (molecular weight 87 kDa).

Filter by Application:

Rabbit anti STAT1 Alpha

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: STAT1 Alpha

Quick Links:

Secondary Antibodies
Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00008 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
	Log in

Rabbit anti Human STAT1 alpha antibody recognizes human signal transducer and activator of transcription 1-alpha also known as transcription factor ISGF-3 components p91/p84. STAT1 alpha is one of two isoforms generated by alternative splicing and encoded for by the STAT1 gene. STAT1 alpha is a ~87 kDa protein belonging to the transcription factor STAT family containing a single SH2 domain. Rabbit anti Human STAT1 alpha antibody recognizes epitopes in a carboxy-terminal region of STAT1 alpha not contained in the short isoform STAT1 beta.

Members of the STAT family respond to cytokines and growth factors and are phosphorylated by receptor associated kinase to form homo- or hetero-dimers. These then translocate to the cell nucleus to act as transcription activators. STAT1 is activated by a number of different ligands including the interferon, gp130 and receptor tyrosine kinase families (Gerhartz et al. 1996). Mutations in the STAT1 gene lead to a number of immunological disorders including severe combined immunodeficiency phenotypes (Sharfe et al. 2014), or more selective immunodeficiencies to macrophagic (usually mycobacteria) bacteria, viruses or fungi depending on the mutation or abnormality involved (Boisson-Dupuis et al. 2012).

Rabbit anti Human STAT1 alpha antibody recognizes human STAT1 alpha as a single band of ~87 kDa in multiple human cell line lysates by western blotting under reducing conditions. It also cross reacts with rodent cell line lysates tested.

Target Species: Human
Western Blotting: Anti STAT1 alpha antibody detects a band of approximately 87 kDa in MCF7 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by ion exchange chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Peptide corresponding to amino acids near the C-terminus of human STAT1 alpha
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.