STAM antibody

Rabbit anti STAM

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
STAM

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VPA00339
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Rabbit anti Human STAM antibody recognizes STAM, also known as signal transducing adapter molecule 1 and HSE1 homolog, signal transducing adapter molecule 1.

The STAM gene encodes a member of the signal-transducing adaptor molecule family. These proteins mediate downstream signaling of cytokine receptors and also play a role in ER to Golgi trafficking by interacting with the coat protein II complex. The STAM gene encoded protein also associates with hepatocyte growth factor-regulated substrate to form the endosomal sorting complex required for transport-0 (ESCRT-0), which sorts ubiquitinated membrane proteins to the ESCRT-1 complex for lysosomal degradation. Alternatively spliced transcript variants have been observed for STAM (provided by RefSeq, Feb 2011).

Rabbit anti Human STAM antibody detects a band of 59 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti STAM detects a band of approximately 59 kDa in Jurkat cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 326-356 of human STAM
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
Q92783
Entrez Gene
STAM
GO Terms
GO:0005829 cytosol
GO:0005070 SH3/SH2 adaptor activity
GO:0006886 intracellular protein transport
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0031901 early endosome membrane
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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