SRSF1 antibody

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Anti SRSF1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with SRSF1 antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 5 second exposure. Arrow points to SRSF1 (molecular weight 33 kDa)

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Rabbit anti SRSF1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: SRSF1

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Rabbit anti Human SRSF1 antibody recognizes the SR splicing factor 1, also known as serine/arginine-rich splicing factor 1, alternative-splicing factor 1 and pre-mRNA-splicing factor SF2.

SRSF1 encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for SRSF1. In addition, a pseudogene of SRSF1 has been found on chromosome 13 (provided by RefSeq, Sep 2010).

Rabbit anti Human SRSF1 antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti SRSF1 antibody recognizes a band of approximately 33 kDa in HEK293 cell lysates
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
2% Sucrose
Immunogen: Synthetic peptide corresponding to the middle segment of human SRSF1
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.