SRC (pTyr530) antibody

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Rabbit anti SRC (pTyr530)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2698
Datasheet
P WB
MSDS
50 µl loader

Rabbit anti SRC (pTyr530) antibody recognizes proto-oncogene tyrosine-protein kinase Src, also known as SRC1, when phosphorylated at tyrosine 530. SRC belongs to the SRC family of protein tyrosine kinases that mediate signal transduction of specific receptor pathways (Thomas, S.M. & Brugge, J.S. 1997). Rabbit anti SRC (pTyr530) is raised against the tyrosine present in conserved EPQyQPG motif. Human tyrosine 530 is commonly referred to and named according to the chicken SRC protein. In chicken, the corresponding residue is tyrosine 527 (Boggon, T.J. & Eck, M.J. 2004). Tyrosine 530 is a negative regulatory site of SRC kinase activity and is phosphorylated by the Src-specific kinase Csk (Nada, S. et al. 1991) or its homolog Chk (Hamaguchi, I. et al. 1996 and Davidson, D. et al. 1997).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to SRC (pTyr530) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 530 of human SRC

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P12931 Related reagents
Entrez Gene
SRC Related reagents
GO Terms
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0005070 SH3/SH2 adaptor activity
GO:0005178 integrin binding
GO:0005524 ATP binding
GO:0005901 caveola
GO:0006468 protein phosphorylation
GO:0007172 signal complex assembly
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007243 intracellular protein kinase cascade
GO:0007265 Ras protein signal transduction
GO:0007411 axon guidance
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0016044 cellular membrane organization
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0033625 positive regulation of integrin activation
GO:0034329 cell junction assembly
GO:0042169 SH2 domain binding
GO:0043114 regulation of vascular permeability
GO:0044419 interspecies interaction between organisms
GO:0045124 regulation of bone resorption
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050900 leukocyte migration
GO:0070555 response to interleukin-1
Regulatory
For research purposes only

Applications of SRC (pTyr530) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml