Rabbit anti SRC (pTyr530) antibody
recognizes proto-oncogene tyrosine-protein kinase Src, also known as SRC1, when phosphorylated at tyrosine 530. SRC belongs to the SRC family of protein tyrosine kinases that mediate signal transduction of specific receptor pathways (Thomas, S.M. & Brugge, J.S. 1997
). Rabbit anti SRC (pTyr530) is raised against the tyrosine present in conserved EPQyQPG motif. Human tyrosine 530 is commonly referred to and named according to the chicken SRC protein. In chicken, the corresponding residue is tyrosine 527 (Boggon, T.J. & Eck, M.J. 2004
). Tyrosine 530 is a negative regulatory site of SRC kinase activity and is phosphorylated by the Src-specific kinase Csk (Nada, S. et al. 1991
) or its homolog Chk (Hamaguchi, I. et al. 1996
and Davidson, D. et al. 1997
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to SRC (pTyr530) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding tyrosine 530 of human SRC
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
non-membrane spanning protein tyrosine kinase activity
SH3/SH2 adaptor activity
signal complex assembly
epidermal growth factor receptor signaling pathway
intracellular protein kinase cascade
Ras protein signal transduction
fibroblast growth factor receptor signaling pathway
cellular membrane organization
T cell costimulation
positive regulation of integrin activation
cell junction assembly
SH2 domain binding
regulation of vascular permeability
interspecies interaction between organisms
regulation of bone resorption
nerve growth factor receptor signaling pathway
response to interleukin-1
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of SRC (pTyr530) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
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