SRC (pTyr530) antibody

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Anti SRC (pTyr530) Antibody thumbnail image 2

Anti SRC (pTyr530) Antibody gallery image 1 — Western blot analysis of extracts from HeLa cells untreated or treated with CIP using Rabbit anti SRC (pTyr530) antibody at a dilution of 1/1000. Cells were treated with CIP (20μl CIP for every 400μl cell lysate) at 37°C for 1 hour. 3% BSA was used for blocking.

Anti SRC (pTyr530) Antibody gallery image 2 — Immunohistochemistry of paraffin-embedded human breast carcinoma using Rabbit anti SRC (pTyr530) antibody

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Rabbit anti SRC (pTyr530)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: SRC (pTyr530)

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AHP2698 Datasheet Datasheet SDS SDS	P WB	50 µl		Log in	Get Quote
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Rabbit anti SRC (pTyr530) antibody recognizes proto-oncogene tyrosine-protein kinase Src, also known as SRC1, when phosphorylated at tyrosine 530. SRC belongs to the SRC family of protein tyrosine kinases that mediate signal transduction of specific receptor pathways (Thomas, S.M. & Brugge, J.S. 1997). Rabbit anti SRC (pTyr530) is raised against the tyrosine present in conserved EPQyQPG motif. Human tyrosine 530 is commonly referred to and named according to the chicken SRC protein. In chicken, the corresponding residue is tyrosine 527 (Boggon, T.J. & Eck, M.J. 2004). Tyrosine 530 is a negative regulatory site of SRC kinase activity and is phosphorylated by the Src-specific kinase Csk (Nada, S. et al. 1991) or its homolog Chk (Hamaguchi, I. et al. 1996 and Davidson, D. et al. 1997).

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse Expected from Sequence; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to SRC (pTyr530) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide
50% Glycerol
Immunogen: Phospho specific-peptide corresponding to residues surrounding tyrosine 530 of human SRC
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Mouse	Expected from Sequence

Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin		1/50	1/100
Western Blotting		1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote
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Description	TidyBlot Western Blot Detection Reagent:HRP

UniProt: P12931
Entrez Gene: SRC
GO Terms: GO:0005829 cytosol; GO:0004715 non-membrane spanning protein tyrosine kinase activity; GO:0005070 SH3/SH2 adaptor activity; GO:0005178 integrin binding; GO:0005524 ATP binding; GO:0005901 caveola; GO:0006468 protein phosphorylation; GO:0007172 signal complex assembly; GO:0007173 epidermal growth factor receptor signaling pathway; View More GO Terms; GO:0007243 intracellular protein kinase cascade; GO:0007265 Ras protein signal transduction; GO:0007411 axon guidance; GO:0008543 fibroblast growth factor receptor signaling pathway; GO:0016044 cellular membrane organization; GO:0030168 platelet activation; GO:0031295 T cell costimulation; GO:0033625 positive regulation of integrin activation; GO:0034329 cell junction assembly; GO:0042169 SH2 domain binding; GO:0043114 regulation of vascular permeability; GO:0044419 interspecies interaction between organisms; GO:0045124 regulation of bone resorption; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050900 leukocyte migration; GO:0070555 response to interleukin-1