Splicing Factor 3B Subunit 2 antibody

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Anti Splicing Factor 3B Subunit 2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with splicing factor 3B subunit 2 antibody followed by detection with Goat anti Rabbit HRP conjugated(1/10,000, STAR208P) and visualized on the ChemiDoc MP with 19 second exposure. Arrow points to splicing factor 3B subunit 2 (molecular weight 140 kDa)

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Rabbit anti Splicing Factor 3B Subunit 2

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Splicing Factor 3B Subunit 2

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Rabbit anti Human splicing factor 3B subunit 2 antibody recognizes the splicing factor 3B subunit 2, also known as SAP 145, pre-mRNA splicing factor SF3b 145 kDa subunit, pre-mRNA-splicing factor SF3b 145 kDa subunit and spliceosome associated protein 145.

Encoded by the SF3B2 gene, splicing factor 3B subunit 2 is a subunit of the splicing factor 3B protein complex. Splicing factor 3B, together with splicing factor 3A and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3B/3A complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3B is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3B complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus (provided by RefSeq, Jul 2008).

Rabbit anti Human Splicing factor 3B subunit 2 antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti splicing factor 3B subunit 2 detects a band of approximately 140 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH conjugated synthetic peptide between 539-568 amino acids from the central region of human splicing factor 3B subunit 2
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q13435
Entrez Gene: SF3B2
GO Terms: GO:0005515 protein binding; GO:0000398 nuclear mRNA splicing, via spliceosome; GO:0003676 nucleic acid binding; GO:0005654 nucleoplasm; GO:0005689 U12-type spliceosomal complex; GO:0044419 interspecies interaction between organisms