Splicing Factor 3B Subunit 2 antibody

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Rabbit anti Splicing Factor 3B Subunit 2

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00250
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human splicing factor 3B subunit 2 antibody recognizes the splicing factor 3B subunit 2, also known as SAP 145, pre-mRNA splicing factor SF3b 145 kDa subunit, pre-mRNA-splicing factor SF3b 145 kDa subunit and spliceosome associated protein 145.

Encoded by the SF3B2 gene, splicing factor 3B subunit 2 is a subunit of the splicing factor 3B protein complex. Splicing factor 3B, together with splicing factor 3A and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3B/3A complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3B is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3B complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus (provided by RefSeq, Jul 2008).

Rabbit anti Human Splicing factor 3B subunit 2 antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti splicing factor 3B subunit 2 detects a band of approximately 140 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 539-568 amino acids from the central region of human splicing factor 3B subunit 2

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q13435 Related reagents
Entrez Gene
SF3B2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003676 nucleic acid binding
GO:0005654 nucleoplasm
GO:0005689 U12-type spliceosomal complex
GO:0044419 interspecies interaction between organisms
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Splicing Factor 3B Subunit 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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