Splicing Factor 3B Subunit 2 antibody
Rabbit anti Human splicing factor 3B subunit 2 antibody recognizes the splicing factor 3B subunit 2, also known as SAP 145, pre-mRNA splicing factor SF3b 145 kDa subunit, pre-mRNA-splicing factor SF3b 145 kDa subunit and spliceosome associated protein 145.
Encoded by the SF3B2 gene, splicing factor 3B subunit 2 is a subunit of the splicing factor 3B protein complex. Splicing factor 3B, together with splicing factor 3A and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3B/3A complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3B is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3B complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus (provided by RefSeq, Jul 2008).
Rabbit anti Human Splicing factor 3B subunit 2 antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the SF3B2 gene, splicing factor 3B subunit 2 is a subunit of the splicing factor 3B protein complex. Splicing factor 3B, together with splicing factor 3A and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3B/3A complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3B is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3B complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus (provided by RefSeq, Jul 2008).
Rabbit anti Human Splicing factor 3B subunit 2 antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti splicing factor 3B subunit 2 detects a band of approximately 140 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide between 539-568 amino acids from the central region of human splicing factor 3B subunit 2
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q13435
- Entrez Gene
- SF3B2
- GO Terms
- GO:0005515 protein binding
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0003676 nucleic acid binding
- GO:0005654 nucleoplasm
- GO:0005689 U12-type spliceosomal complex
- GO:0044419 interspecies interaction between organisms
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Splicing Factor 3B Subunit 2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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