SPHK1 antibody

Rabbit anti Human SPHK1 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
SPHK1
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1162
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P * 50 µg loader
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Rabbit anti Human SphK1 antibody detects epitopes within the N-terminal region human SphK1, also known sphingosine kinase 1. SphK1 catalyzes the phosphorylation of sphingosine 1-phosphate; an important lipid mediator with multiple functions. In light of this, SphK1 has been implicated in regulation of Ca2+ homoeostasis, inflammation, immunity and cancer growth. However, its central function is thought to be in the development of the cardiovascular and central nervous system. SphK1 has a molecular weight of ~42 kDa.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to SphK1 were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the N-terminal domain of human SphK1, conjugated to KLH.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 5 15ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Adrenal, Pheochromocytes.

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References for SPHK1 antibody

  1. Maceyka, M. et al. (2005) SphK1 and SphK2, sphingosine kinase isoenzymes with opposing functions in sphingolipid metabolism.
    J Biol Chem. 280 (44): 37118-29.
  2. Alemany, R. et al. (2007) Regulation and functional roles of sphingosine kinases.
    Naunyn Schmiedebergs Arch Pharmacol. 374 (5-6): 413-28.

Synonyms
Sphingosine Kinase 1
RRID
AB_808545
UniProt
Q9NYA1
Entrez Gene
SPHK1
GO Terms
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0004143 diacylglycerol kinase activity
GO:0005829 cytosol
GO:0005516 calmodulin binding
GO:0005524 ATP binding
GO:0005624 membrane fraction
GO:0005625 soluble fraction
GO:0006670 sphingosine metabolic process
GO:0006916 anti-apoptosis
GO:0007205 activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway
GO:0019722 calcium-mediated signaling
GO:0008481 sphinganine kinase activity
GO:0045987 positive regulation of smooth muscle contraction
GO:0010803 regulation of tumor necrosis factor-mediated signaling pathway
GO:0017050 D-erythro-sphingosine kinase activity
GO:0030307 positive regulation of cell growth
GO:0030335 positive regulation of cell migration
GO:0031398 positive regulation of protein ubiquitination
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0045766 positive regulation of angiogenesis
GO:0045931 positive regulation of mitotic cell cycle
GO:0046521 sphingoid catabolic process
GO:0048146 positive regulation of fibroblast proliferation
GO:0051084 'de novo' posttranslational protein folding
GO:0051092 positive regulation of NF-kappaB transcription factor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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