SPARC antibody

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Rabbit anti SPARC

Rabbit anti-human SPARC antibody recognizes SPARC, also known as BM-40, basement-membrane protein 40, cysteine-rich protein, osteonectin or secreted protein acidic and rich in cysteine. This antibody has been extensively validated for western blotting using whole cell lysates.

SPARC Antibody with Control Lysate

Rabbit anti-human SPARC antibody recognizes SPARC, also known as BM-40, basement-membrane protein 40, cysteine-rich protein, osteonectin or secreted protein acidic and rich in cysteine. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00564 WB 100 µl
VPA00564KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human SPARC antibody recognizes SPARC, also known as BM-40, basement-membrane protein 40, osteonectin or secreted protein acidic and rich in cysteine.

The SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion (provided by RefSeq, Dec 2011). Mutation of the SPARC gene causes presentation of Osteogenesis imperfecta 17 (OI17), an autosomal recessive form of osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma (Mendoza-Londono et al. 2015).

Rabbit anti Human SPARC antibody detects a band of 35 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the C terminal region of human SPARC

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P09486 Related reagents
Entrez Gene
SPARC Related reagents
GO Terms
GO:0001503 ossification
GO:0005509 calcium ion binding
GO:0002576 platelet degranulation
GO:0005518 collagen binding
GO:0005604 basement membrane
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of SPARC antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti SPARC detects a band of approximately 35 kDa in HEK293 cell lysates
Western Blotting
Anti SPARC detects a band of approximately 35 kDa in HEK293 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB