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Sp1 antibody

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Anti Sp1 Antibody gallery image 1
Western blotting analysis of Sp1 expression in NIH/3T3 cell lysates using Rabbit anti Sp1 antibody (AHP2414) at a 1/200 dilution.

Rabbit anti Sp1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2414
Datasheet
WB
MSDS
0.1 mg loader

Rabbit anti Sp1 antibody recognizes specificity protein 1 (Sp1). Sp1 acts as a transcription factor and has been identified as a member of the Sp/Krüppel-like transcription factor family, playing a role in cancer metabolism (Archer 2011).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Antiserum Preparation
Antiserum to Sp1 was raised by repeated immunisation of rabbits with highly purified antigen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.2% Gelatin
Immunogen
Peptide mapping to an internal domain of human Sp1
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P08047
Entrez Gene
SP1
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003702 RNA polymerase II transcription factor activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0008022 protein C-terminus binding
GO:0008134 transcription factor binding
GO:0008270 zinc ion binding
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0042803 protein homodimerization activity
GO:0042826 histone deacetylase binding
GO:0043923 positive regulation by host of viral transcription
GO:0045817 positive regulation of transcription from RNA polymerase II promoter, global
Regulatory
For research purposes only

Applications of Sp1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti Sp1 recognizes a band of approximately 100 kDa when run under reducing conditions
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
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