Sp1 antibody
Rabbit anti Sp1 antibody recognizes specificity protein 1 (Sp1). Sp1 acts as a transcription factor and has been identified as a member of the Sp/Krüppel-like transcription factor family, playing a role in cancer metabolism (Archer 2011).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Antiserum Preparation
- Antiserum to Sp1 was raised by repeated immunisation of rabbits with highly purified antigen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
0.2% Gelatin - Immunogen
- Peptide mapping to an internal domain of human Sp1
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P08047
- Entrez Gene
- SP1
- GO Terms
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003702 RNA polymerase II transcription factor activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0008022 protein C-terminus binding
- GO:0008134 transcription factor binding
- GO:0008270 zinc ion binding
- GO:0010843 promoter binding
- GO:0016563 transcription activator activity
- GO:0042803 protein homodimerization activity
- GO:0042826 histone deacetylase binding
- GO:0043923 positive regulation by host of viral transcription
- GO:0045817 positive regulation of transcription from RNA polymerase II promoter, global
- Regulatory
- For research purposes only
Applications of Sp1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti Sp1 recognizes a band of approximately 100 kDa when run under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Fluorescent Spectraviewer
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- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
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- Select combined or multi-laser view to visualize the spectra
