SOX2 antibody

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Rabbit anti SOX2

SOX2 antibody recognizes the transcription factor SOX-2, also known as SRY-related HMG-box gene 2. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00238 WB 100 µl
Rabbit anti Human SOX2 antibody recognizes the transcription factor SOX2, also known as SRY-related HMG-box gene 2.

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. SOX2 is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in the SOX2 gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. SOX2 lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT) (provided by RefSeq, Jul 2008).

Rabbit anti Human SOX2 antibody detects a band of 35 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti SOX2 detects a band of approximately 35 kDa in NTERA-2 cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 282-316 amino acids from the C-terminal region of human SOX2
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P48431 Related reagents
Entrez Gene
SOX2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0001649 osteoblast differentiation
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005829 cytosol
GO:0005667 transcription factor complex
GO:0006325 chromatin organization
GO:0007050 cell cycle arrest
GO:0009611 response to wounding
GO:0010843 promoter binding
GO:0021781 glial cell fate commitment
GO:0035019 somatic stem cell maintenance
GO:0035198 miRNA binding
GO:0043281 regulation of caspase activity
GO:0043410 positive regulation of MAPKKK cascade
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0050680 negative regulation of epithelial cell proliferation
GO:0070848 response to growth factor stimulus
GO:0090090 negative regulation of canonical Wnt receptor signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of SOX2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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