SNRNP200 antibody
Rabbit anti SNRNP200
Rabbit anti human SNRNP200 antibody recognizes U5 small nuclear ribonucleoprotein 200 kDa helicase, also known as BRR2 homolog, U5 snRNP-specific 200 kDa protein, activating signal cointegrator 1 complex subunit 3-like 1 or ASCC3L1. This antibody has been extensively validated for western blotting.
SNRNP200 Antibody with Control Lysate
Rabbit anti human SNRNP200 antibody recognizes U5 small nuclear ribonucleoprotein 200 kDa helicase, also known as BRR2 homolog, U5 snRNP-specific 200 kDa protein, activating signal cointegrator 1 complex subunit 3-like 1 or ASCC3L1.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00395 | WB | 100 µl | ||||
| VPA00395KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human SNRNP200 antibody recognizes SNRNP200, also known as BRR2 homolog, U5 snRNP-specific 200 kDa protein, activating signal cointegrator 1 complex subunit 3-like 1 and ASCC3L1.
Pre-mRNA splicing is catalyzed by the spliceosome, a complex of specialized RNA and protein subunits that removes introns from a transcribed pre-mRNA segment. The spliceosome consists of small nuclear RNA proteins (snRNPs) U1, U2, U4, U5 and U6, together with approximately 80 conserved proteins. U5 snRNP contains nine specific proteins. The SNRNP200 gene encodes one of the U5 snRNP-specific proteins. This protein belongs to the DEXH-box family of putative RNA helicases. It is a core component of U4/U6-U5 snRNPs and appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices. Mutations in SNRNP200 cause autosomal-dominant retinitis pigmentosa. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined (provided by RefSeq, Mar 2010).
Rabbit anti Human SNRNP200 antibody detects a band of 245 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Pre-mRNA splicing is catalyzed by the spliceosome, a complex of specialized RNA and protein subunits that removes introns from a transcribed pre-mRNA segment. The spliceosome consists of small nuclear RNA proteins (snRNPs) U1, U2, U4, U5 and U6, together with approximately 80 conserved proteins. U5 snRNP contains nine specific proteins. The SNRNP200 gene encodes one of the U5 snRNP-specific proteins. This protein belongs to the DEXH-box family of putative RNA helicases. It is a core component of U4/U6-U5 snRNPs and appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices. Mutations in SNRNP200 cause autosomal-dominant retinitis pigmentosa. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined (provided by RefSeq, Mar 2010).
Rabbit anti Human SNRNP200 antibody detects a band of 245 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti SNRNP200 detects a band of approximately 245 kDa in HEK293 cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide corresponding to the N-terminal region of human SNRNP200
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- O75643 Related reagents
- Entrez Gene
- SNRNP200 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0000354 cis assembly of pre-catalytic spliceosome
- GO:0003676 nucleic acid binding
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0005681 spliceosomal complex
- GO:0005682 U5 snRNP
- GO:0007601 visual perception
- GO:0008026 ATP-dependent helicase activity
- GO:0050896 response to stimulus
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of SNRNP200 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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