SNAP-25 antibody

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Anti SNAP-25 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell and brain lysates probed with SNAP-25 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 5 second exposure. Arrow points to SNAP-25 (molecular weight 25 kDa)

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Goat anti SNAP-25

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: SNAP-25

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Goat anti Human SNAP-25 antibody recognizes synaptosomal-associated protein 25.

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by SNAP25 contributes the other two. Therefore, SNAP25 product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for SNAP25 (provided by RefSeq, Jul 2008).

Goat anti Human SNAP-25 antibody detects a band of 25 kDa. The antibody has been extensively validated for western blotting using brain lysates.

Target Species: Human
Western Blotting: Anti SNAP-25 detects a band of approximately 25 kDa in mouse brain lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃) 0.5 % BSA
Immunogen: Peptide with the sequence C-DEANQRATKMLGSG, from the C terminus of the protein sequence
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.