SMURF1 antibody

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Rabbit anti SMURF1

SMURF1 antibody recognizes E3 ubiquitin-protein ligase SMURF1, also known as SMAD-specific E3 ubiquitin-protein ligase 1, Smad ubiquitination regulatory factor 1 or hSMURF1. This antibody has been extensively validated for western blotting using whole cell lysates.

SMURF1 Antibody with Control Lysate

SMURF1 antibody recognizes E3 ubiquitin-protein ligase SMURF1, also known as SMAD-specific E3 ubiquitin-protein ligase 1, Smad ubiquitination regulatory factor 1 or hSMURF1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00234 WB 100 µl
VPA00234KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human SMURF1 antibody recognizes the E3 ubiquitin-protein ligase SMURF1, also known as SMAD-specific E3 ubiquitin-protein ligase 1, Smad ubiquitination regulatory factor 1 and hSMURF1.

The SMURF1 gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Dec 2010).

Rabbit anti Human SMURF1 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti SMURF1 detects a band of approximately 81 kDa in Ramos cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Preparation
20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 711-740 amino acids from the C-terminal region of human SMURF1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Ramos lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9HCE7 Related reagents
Entrez Gene
SMURF1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000209 protein polyubiquitination
GO:0042787 protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0005829 cytosol
GO:0004842 ubiquitin-protein ligase activity
GO:0006611 protein export from nucleus
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007398 ectoderm development
GO:0030154 cell differentiation
GO:0030509 BMP signaling pathway
GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway
GO:0030514 negative regulation of BMP signaling pathway
GO:0030579 ubiquitin-dependent SMAD protein catabolic process
GO:0031398 positive regulation of protein ubiquitination
GO:0032801 receptor catabolic process
GO:0034394 protein localization at cell surface
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0048185 activin binding
GO:0070411 I-SMAD binding
GO:0070412 R-SMAD binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of SMURF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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