SMURF1 antibody

100% Secure

Rabbit anti SMURF1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VPA00234 Datasheet	WB SDS	100 µl	Log in		Get Quote

Rabbit anti Human SMURF1 antibody recognizes the E3 ubiquitin-protein ligase SMURF1, also known as SMAD-specific E3 ubiquitin-protein ligase 1, Smad ubiquitination regulatory factor 1 and hSMURF1.

The SMURF1 gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Dec 2010).

Rabbit anti Human SMURF1 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti SMURF1 detects a band of approximately 81 kDa in Ramos cell lysates
Product Form: Purified IgG - liquid
Preparation: Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: KLH conjugated synthetic peptide between 711-740 amino acids from the C-terminal region of human SMURF1

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: 12 months from date of despatch

More Information

UniProt: Q9HCE7
Entrez Gene: SMURF1
GO Terms: GO:0005886 plasma membrane; GO:0000209 protein polyubiquitination; GO:0042787 protein ubiquitination involved in ubiquitin-dependent protein catabolic process; GO:0005829 cytosol; GO:0004842 ubiquitin-protein ligase activity; GO:0006611 protein export from nucleus; GO:0007179 transforming growth factor beta receptor signaling pathway; GO:0007398 ectoderm development; GO:0030154 cell differentiation; GO:0030509 BMP signaling pathway; GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway; GO:0030514 negative regulation of BMP signaling pathway; GO:0030579 ubiquitin-dependent SMAD protein catabolic process; GO:0031398 positive regulation of protein ubiquitination; GO:0032801 receptor catabolic process; GO:0034394 protein localization at cell surface; GO:0043161 proteasomal ubiquitin-dependent protein catabolic process; GO:0048185 activin binding; GO:0070411 I-SMAD binding; GO:0070412 R-SMAD binding
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of SMURF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in		Get Quote

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer