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SMURF1 antibody

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Western Blot Validated
Anti SMURF1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with SMURF1 antibody followed by detection with Goat anti Rabbit HRP conjugated(1/10,000,STAR208P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to SMURF1 (molecular weight 81 kDa)

Rabbit anti SMURF1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00234
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human SMURF1 antibody recognizes the E3 ubiquitin-protein ligase SMURF1, also known as SMAD-specific E3 ubiquitin-protein ligase 1, Smad ubiquitination regulatory factor 1 and hSMURF1.

The SMURF1 gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Dec 2010).

Rabbit anti Human SMURF1 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti SMURF1 detects a band of approximately 81 kDa in Ramos cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 711-740 amino acids from the C-terminal region of human SMURF1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9HCE7
Entrez Gene
SMURF1
GO Terms
GO:0005886 plasma membrane
GO:0000209 protein polyubiquitination
GO:0042787 protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0005829 cytosol
GO:0004842 ubiquitin-protein ligase activity
GO:0006611 protein export from nucleus
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007398 ectoderm development
GO:0030154 cell differentiation
GO:0030509 BMP signaling pathway
GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway
GO:0030514 negative regulation of BMP signaling pathway
GO:0030579 ubiquitin-dependent SMAD protein catabolic process
GO:0031398 positive regulation of protein ubiquitination
GO:0032801 receptor catabolic process
GO:0034394 protein localization at cell surface
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0048185 activin binding
GO:0070411 I-SMAD binding
GO:0070412 R-SMAD binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of SMURF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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