SMURF1 antibody
Rabbit anti SMURF1
SMURF1 antibody recognizes E3 ubiquitin-protein ligase SMURF1, also known as SMAD-specific E3 ubiquitin-protein ligase 1, Smad ubiquitination regulatory factor 1 or hSMURF1. This antibody has been extensively validated for western blotting using whole cell lysates.
SMURF1 Antibody with Control Lysate
SMURF1 antibody recognizes E3 ubiquitin-protein ligase SMURF1, also known as SMAD-specific E3 ubiquitin-protein ligase 1, Smad ubiquitination regulatory factor 1 or hSMURF1. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00234 | WB | 100 µl | ||||
| VPA00234KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human SMURF1 antibody recognizes the E3 ubiquitin-protein ligase SMURF1, also known as SMAD-specific E3 ubiquitin-protein ligase 1, Smad ubiquitination regulatory factor 1 and hSMURF1.
The SMURF1 gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Dec 2010).
Rabbit anti Human SMURF1 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The SMURF1 gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Dec 2010).
Rabbit anti Human SMURF1 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti SMURF1 detects a band of approximately 81 kDa in Ramos cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Preparation
- 20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide between 711-740 amino acids from the C-terminal region of human SMURF1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Ramos lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q9HCE7 Related reagents
- Entrez Gene
- SMURF1 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0000209 protein polyubiquitination
- GO:0042787 protein ubiquitination involved in ubiquitin-dependent protein catabolic process
- GO:0005829 cytosol
- GO:0004842 ubiquitin-protein ligase activity
- GO:0006611 protein export from nucleus
- GO:0007179 transforming growth factor beta receptor signaling pathway
- GO:0007398 ectoderm development
- GO:0030154 cell differentiation
- GO:0030509 BMP signaling pathway
- GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway
- GO:0030514 negative regulation of BMP signaling pathway
- GO:0030579 ubiquitin-dependent SMAD protein catabolic process
- GO:0031398 positive regulation of protein ubiquitination
- GO:0032801 receptor catabolic process
- GO:0034394 protein localization at cell surface
- GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
- GO:0048185 activin binding
- GO:0070411 I-SMAD binding
- GO:0070412 R-SMAD binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of SMURF1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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