SMURF1 antibody
Rabbit anti SMURF1 antibody recognizes E3 ubiquitin-protein ligase SMURF1, also known as hSMURF1, HECT-type E3 ubiquitin transferase SMURF1 and SMAD ubiquitination regulatory factor 1. It belongs to the HECT family of E3 ubiquitin ligases and controls osteoblast activity and bone homeostasis (Yamashita et al. 2005). It is involved in BMP receptor trafficking and signaling (Murakami and Etlinger 2018). SMURF1 plays a role in breast cancer migration and invasion by downregulating RhoA (Kwon et al. 2013). It also regulates cell migration by ubiquitinating tumor necrosis factor receptor-associated factor 4 (TRAF4) (Wang et al. 2013).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to SMURF1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- A synthetic peptide corresponding to amino acids 66-96 of human SMURF1
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9HCE7
- Entrez Gene
- SMURF1
- GO Terms
- GO:0005886 plasma membrane
- GO:0000209 protein polyubiquitination
- GO:0042787 protein ubiquitination involved in ubiquitin-dependent protein catabolic process
- GO:0005829 cytosol
- GO:0004842 ubiquitin-protein ligase activity
- GO:0006611 protein export from nucleus
- GO:0007179 transforming growth factor beta receptor signaling pathway
- GO:0007398 ectoderm development
- GO:0030154 cell differentiation
- GO:0030509 BMP signaling pathway
- GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway
- GO:0030514 negative regulation of BMP signaling pathway
- GO:0030579 ubiquitin-dependent SMAD protein catabolic process
- GO:0031398 positive regulation of protein ubiquitination
- GO:0032801 receptor catabolic process
- GO:0034394 protein localization at cell surface
- GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
- GO:0048185 activin binding
- GO:0070411 I-SMAD binding
- GO:0070412 R-SMAD binding
- Regulatory
- For research purposes only
Applications of SMURF1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti SMURF1 antibody detects a band of approximately 86 kDa in HeLa cell lysate and mouse kidney lysate under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
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