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SMURF1 antibody

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Anti SMURF1 Antibody gallery image 1
Western blot analysis of HeLa cell lysate and mouse kidney tissue lysate using Rabbit anti SMURF1 antibody. 3% non-fat dry milk in TBST was used for blocking

Rabbit anti SMURF1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2740
Datasheet
WB
SDS
50 µl loader

Rabbit anti SMURF1 antibody recognizes E3 ubiquitin-protein ligase SMURF1, also known as hSMURF1, HECT-type E3 ubiquitin transferase SMURF1 and SMAD ubiquitination regulatory factor 1. It belongs to the HECT family of E3 ubiquitin ligases and controls osteoblast activity and bone homeostasis (Yamashita et al. 2005). It is involved in BMP receptor trafficking and signaling (Murakami and Etlinger 2018). SMURF1 plays a role in breast cancer migration and invasion by downregulating RhoA (Kwon et al. 2013). It also regulates cell migration by ubiquitinating tumor necrosis factor receptor-associated factor 4 (TRAF4) (Wang et al. 2013).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to SMURF1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
A synthetic peptide corresponding to amino acids 66-96 of human SMURF1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9HCE7
Entrez Gene
SMURF1
GO Terms
GO:0005886 plasma membrane
GO:0000209 protein polyubiquitination
GO:0042787 protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0005829 cytosol
GO:0004842 ubiquitin-protein ligase activity
GO:0006611 protein export from nucleus
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007398 ectoderm development
GO:0030154 cell differentiation
GO:0030509 BMP signaling pathway
GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway
GO:0030514 negative regulation of BMP signaling pathway
GO:0030579 ubiquitin-dependent SMAD protein catabolic process
GO:0031398 positive regulation of protein ubiquitination
GO:0032801 receptor catabolic process
GO:0034394 protein localization at cell surface
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0048185 activin binding
GO:0070411 I-SMAD binding
GO:0070412 R-SMAD binding
Regulatory
For research purposes only

Applications of SMURF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti SMURF1 antibody detects a band of approximately 86 kDa in HeLa cell lysate and mouse kidney lysate under reducing conditions
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

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