Rabbit anti SMURF1 antibody
recognizes E3 ubiquitin-protein ligase SMURF1, also known as hSMURF1, HECT-type E3 ubiquitin transferase SMURF1 and SMAD ubiquitination regulatory factor 1. It belongs to the HECT family of E3 ubiquitin ligases and controls osteoblast activity and bone homeostasis (Yamashita et al. 2005
). It is involved in BMP receptor trafficking and signaling (Murakami and Etlinger 2018
). SMURF1 plays a role in breast cancer migration and invasion by downregulating RhoA (Kwon et al. 2013
). It also regulates cell migration by ubiquitinating tumor necrosis factor receptor-associated factor 4 (TRAF4) (Wang et al. 2013
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to SMURF1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- A synthetic peptide corresponding to amino acids 66-96 of human SMURF1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
ubiquitin-protein ligase activity
protein export from nucleus
transforming growth factor beta receptor signaling pathway
BMP signaling pathway
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
ubiquitin-dependent SMAD protein catabolic process
positive regulation of protein ubiquitination
receptor catabolic process
protein localization at cell surface
proteasomal ubiquitin-dependent protein catabolic process
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of SMURF1 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti SMURF1 antibody detects a band of approximately 86 kDa in HeLa cell lysate and mouse kidney lysate under reducing conditions
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Secondary Antibodies Available