SMAD6 antibody

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Rabbit anti SMAD6

Rabbit anti human SMAD6 antibody recognizes mothers against decapentaplegic homolog 6, also known as MAD homolog 6, SMAD or mothers against DPP homolog 6. This antibody has been extensively validated for western blotting using whole cell lysates.

SMAD6 Antibody with Control Lysate

Rabbit anti human SMAD6 antibody recognizes mothers against decapentaplegic homolog 6, also known as MAD homolog 6, SMAD or mothers against DPP homolog 6. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00295 WB 100 µl
VPA00295KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human SMAD6 antibody recognizes SMAD6, also known as MAD homolog 6, SMAD and mothers against decapentaplegic homolog 6.

The protein encoded by the SMAD6 gene belongs to the SMAD family of proteins, which are related to Drosophila “mothers against decapentaplegic” (Mad) and C. elegans “Sma”. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants encoding different isoforms have been found for SMAD6 (provided by RefSeq, May 2009).

Rabbit anti SMAD6 antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti SMAD6 detects a band of approximately 62 kDa in Jurkat cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 358-386 of human SMAD6
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
O43541 Related reagents
Entrez Gene
SMAD6 Related reagents
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005829 cytosol
GO:0030617 transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity
GO:0005667 transcription factor complex
GO:0006355 regulation of transcription, DNA-dependent
GO:0006955 immune response
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007352 zygotic specification of dorsal/ventral axis
GO:0010991 negative regulation of SMAD protein complex assembly
GO:0030509 BMP signaling pathway
GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway
GO:0030514 negative regulation of BMP signaling pathway
GO:0031625 ubiquitin protein ligase binding
GO:0034616 response to laminar fluid shear stress
GO:0034713 type I transforming growth factor beta receptor binding
GO:0043066 negative regulation of apoptosis
GO:0043154 negative regulation of caspase activity
GO:0045750 positive regulation of S phase of mitotic cell cycle
GO:0060394 negative regulation of pathway-restricted SMAD protein phosphorylation
GO:0070410 co-SMAD binding
GO:0070411 I-SMAD binding
GO:0070412 R-SMAD binding
GO:0070698 type I activin receptor binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of SMAD6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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