SMAD4 antibody

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Rabbit anti SMAD4

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2521 WB 50 µl
Rabbit anti SMAD4 antibody recognizes SMAD4, also known as mothers against decapentaplegic homolog 4 and deletion target in pancreatic carcinoma 4. SMAD4 is a transcription factor and belongs to the dwarfin/SMAD family. SMAD4 is categorized as the common mediator SMAD (co-SMAD); it associates with receptor-regulated SMADS (SMAD1, SMAD2, SMAD3, SMAD5 and SMAD8) and plays a key role in the TGF-beta signaling pathway.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to SMAD4 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant human SMAD4

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q13485 Related reagents
Entrez Gene
SMAD4 Related reagents
GO Terms
GO:0001666 response to hypoxia
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005829 cytosol
GO:0030616 transforming growth factor beta receptor, common-partner cytoplasmic mediator activity
GO:0005813 centrosome
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007183 SMAD protein complex assembly
GO:0010718 positive regulation of epithelial to mesenchymal transition
GO:0010843 promoter binding
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0030308 negative regulation of cell growth
GO:0030509 BMP signaling pathway
GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
GO:0032444 activin responsive factor complex
GO:0032909 regulation of transforming growth factor-beta2 production
GO:0042177 negative regulation of protein catabolic process
GO:0042803 protein homodimerization activity
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0060021 palate development
GO:0060391 positive regulation of SMAD protein import into nucleus
GO:0060395 SMAD protein signal transduction
GO:0070411 I-SMAD binding
GO:0070412 R-SMAD binding
Regulatory
For research purposes only

Applications of SMAD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti SMAD4 antibody detects a band of approximately 60 kDa in cell lysates under reducing conditions

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*