Rabbit anti SMAD4 antibody recognizes SMAD4, also known as mothers against decapentaplegic homolog 4 and deletion target in pancreatic carcinoma 4. SMAD4 is a transcription factor and belongs to the dwarfin/SMAD family. SMAD4 is categorized as the common mediator SMAD (co-SMAD); it associates with receptor-regulated SMADS (SMAD1, SMAD2, SMAD3, SMAD5 and SMAD8) and plays a key role in the TGF-beta signaling pathway.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to SMAD4 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant human SMAD4
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
response to hypoxia
sequence-specific DNA binding transcription factor activity
transforming growth factor beta receptor, common-partner cytoplasmic mediator activity
transforming growth factor beta receptor signaling pathway
SMAD protein complex assembly
positive regulation of epithelial to mesenchymal transition
positive regulation of pathway-restricted SMAD protein phosphorylation
negative regulation of cell growth
BMP signaling pathway
positive regulation of transforming growth factor beta receptor signaling pathway
activin responsive factor complex
regulation of transforming growth factor-beta2 production
negative regulation of protein catabolic process
protein homodimerization activity
negative regulation of transcription, DNA-dependent
positive regulation of SMAD protein import into nucleus
SMAD protein signal transduction
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of SMAD4 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti SMAD4 antibody detects a band of approximately 60 kDa in cell lysates under reducing conditions
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Secondary Antibodies Available
Useful Reagents Available