SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425) antibody

Rabbit anti SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425)

Product Type
Polyclonal Antibody
Polyclonal IgG
SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425)

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 50 µl loader
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Rabbit anti SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425) antibody recognizes mothers against decapentaplegic homolog 2 (SMAD2), when phosphorylated at serine 465 and serine 467 and mothers against decapentaplegic homolog 3 (SMAD3) when phosphorylated at serine 423 and serine 425. The SMAD protein family mediates transforming growth factor-beta (TGF-β) signaling from the membrane to the nucleus (Attisano, L. & Wrana, J.L. 2002). SMAD2 is phosphorylated on serine 465 and serine 467 by TGF beta type I receptor (TbetaRI). This phosphorylation is essential for SMAD2’s binding to SMAD4 and for the orchestration of TGF beta signaling (Abdollah, S. et al. 1997). The antibody also recognizes SMAD3 phosphorylated on Ser423 and Ser425 by homology.
The TGF beta signaling pathway is involved in many cellular processes including cell growth, differentiation and proliferation. The pathway is deregulated in various diseases, including cancer.

Target Species
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Phospho specific-peptide corresponding to residues surrounding serine 465/467 of human SMAD2 and serine 423/425 of human SMAD3
For research purposes only
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
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Description TidyBlot Western Blot Detection Reagent:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti SMAD3 VPA00166 WB 100 µl loader
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Description Rabbit anti SMAD3
Rabbit anti SMAD2 VPA00182 WB 100 µl loader
List Price Your Price
Description Rabbit anti SMAD2

Entrez Gene
GO Terms
GO:0001707 mesoderm formation
GO:0003690 double-stranded DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005829 cytosol
GO:0035556 intracellular signal transduction
GO:0030618 transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007182 common-partner SMAD protein phosphorylation
GO:0007183 SMAD protein complex assembly
GO:0007352 zygotic specification of dorsal/ventral axis
GO:0008134 transcription factor binding
GO:0009952 anterior/posterior pattern formation
GO:0010718 positive regulation of epithelial to mesenchymal transition
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0017015 regulation of transforming growth factor beta receptor signaling pathway
GO:0031053 primary microRNA processing
GO:0031625 ubiquitin protein ligase binding
GO:0032444 activin responsive factor complex
GO:0034713 type I transforming growth factor beta receptor binding
GO:0043193 positive regulation of gene-specific transcription
GO:0045165 cell fate commitment
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048340 paraxial mesoderm morphogenesis
GO:0051098 regulation of binding
GO:0060021 palate development
GO:0070410 co-SMAD binding
GO:0070411 I-SMAD binding
GO:0070412 R-SMAD binding
GO:0070723 response to cholesterol



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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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