SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425) antibody

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Anti SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425) Antibody gallery image 1 — Western blot analysis of extracts from HeLa cells untreated or treated with TGF-β using Rabbit anti SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425) antibody at a 1/1000 dilution. Cells were cultured in serum free media overnight before treatment with TGF-β (10ng/ml) for 30 minutes. 3% BSA was used for blocking

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Rabbit anti SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425)

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AHP2700 Datasheet Datasheet SDS SDS	WB	50 µl		Log in	Get Quote
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Rabbit anti SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425) antibody recognizes mothers against decapentaplegic homolog 2 (SMAD2), when phosphorylated at serine 465 and serine 467 and mothers against decapentaplegic homolog 3 (SMAD3) when phosphorylated at serine 423 and serine 425. The SMAD protein family mediates transforming growth factor-beta (TGF-β) signaling from the membrane to the nucleus (Attisano, L. & Wrana, J.L. 2002). SMAD2 is phosphorylated on serine 465 and serine 467 by TGF beta type I receptor (TbetaRI). This phosphorylation is essential for SMAD2’s binding to SMAD4 and for the orchestration of TGF beta signaling (Abdollah, S. et al. 1997). The antibody also recognizes SMAD3 phosphorylated on Ser423 and Ser425 by homology.
The TGF beta signaling pathway is involved in many cellular processes including cell growth, differentiation and proliferation. The pathway is deregulated in various diseases, including cancer.

Target Species: Human
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide
50% Glycerol
Immunogen: Phospho specific-peptide corresponding to residues surrounding serine 465/467 of human SMAD2 and serine 423/425 of human SMAD3
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description	Rabbit anti SMAD2

UniProt: Q15796; P84022
Entrez Gene: SMAD3; SMAD2
GO Terms: GO:0001707 mesoderm formation; GO:0003690 double-stranded DNA binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0005829 cytosol; GO:0035556 intracellular signal transduction; GO:0030618 transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; GO:0007179 transforming growth factor beta receptor signaling pathway; GO:0007182 common-partner SMAD protein phosphorylation; GO:0007183 SMAD protein complex assembly; View More GO Terms; GO:0007352 zygotic specification of dorsal/ventral axis; GO:0008134 transcription factor binding; GO:0009952 anterior/posterior pattern formation; GO:0010718 positive regulation of epithelial to mesenchymal transition; GO:0010843 promoter binding; GO:0016563 transcription activator activity; GO:0017015 regulation of transforming growth factor beta receptor signaling pathway; GO:0031053 primary microRNA processing; GO:0031625 ubiquitin protein ligase binding; GO:0032444 activin responsive factor complex; GO:0034713 type I transforming growth factor beta receptor binding; GO:0043193 positive regulation of gene-specific transcription; GO:0045165 cell fate commitment; GO:0045892 negative regulation of transcription, DNA-dependent; GO:0045944 positive regulation of transcription from RNA polymerase II promoter; GO:0048340 paraxial mesoderm morphogenesis; GO:0051098 regulation of binding; GO:0060021 palate development; GO:0070410 co-SMAD binding; GO:0070411 I-SMAD binding; GO:0070412 R-SMAD binding; GO:0070723 response to cholesterol